两种溪黄草转录组的构建及比较分析  

Construction and Comparative Analysis of Transcriptome Data of Two Kinds of Isodon serra

作　　者：黄珊珊[1] 钟华 方春生[1] 余永红[1] 宋卉[1] HUANG Shanshan;ZHONG Hua;FANG Chunsheng;YU Yonghong;SONG Hui(Guangdong Food and Drug Vocational College,Guangzhou 510520,China)

机构地区：[1]广东食品药品职业学院,广州510520

出　　处：《种子》2024年第3期82-89,共8页Seed

基　　金：广东省科技创新战略专项资金(大学生科技创新培育)立项项目“岭南特色中药溪黄草种质分子鉴定研究”(pdjh2023b0879);广东省中药局项目(20222150)。

摘　　要：中药溪黄草药用成分复杂多样,有效成分尚不明确,且分子水平相关研究十分薄弱。因此,构建溪黄草转录组数据库,以生物信息学手段进行分析,可为中药溪黄草药物的研究开发利用奠定基础。本研究利用高通量测序平台Illumina构建了两种常用溪黄草基源植物溪黄草和线纹香茶菜的转录组数据库,并进行了比较分析。两种溪黄草分别获得了144650个和103221个Contigs,N50值分别为1400 bp和1516 bp。这些Contigs聚集成107777个和68220个Unigenes,其功能注释分析以鉴定有效药物成分的基因为主。整个转录组数据中分别有14138个和11756个EST-SSR基因序列。本研究不仅快速地解读了溪黄草整体的基因组信息,还为挖掘相关的功能基因、阐明活性成分的生物合成及其调控机制、评估种质资源和发现隐藏的药用部位提供重要的科学依据。The medicinal components of Isodon serra were complex and diverse,the effective components were not clear,and the related research at molecular level was very weak.Therefore,constructing the transcriptome database and analyzing it by bioinformatics means could lay a foundation for the research,development and utilization of traditional Chinese medicine.In this study,transcriptome databases of two commonly used Isodon serra source plants and Isodon lophanthoides was conducted,using high-throughput sequencing platform Ilumina,and comparative analysis were conducted.A total of 144650 Contigs and 103221 Contigs were obtained from the two species,with N50 values of 1400 bp and 1516 bp,respectively.These Contigs were aggregated into 107777 and 68220 Unigenes,whose functional annotation analysis focused on the identification of genes and active drug ingredients.There were 14138 and 11756 EST-SSR gene sequences in the transcriptome data,respectively.This study not only quickly interpretted the whole genomic information of Isodon serra,but also provided important scientific basis for mining related functional genes,elucidating biosynthesis of active components and their regulatory mechanisms,evaluating germplasm resources and discovering hidden medicinal sites.

关 键 词：溪黄草 转录组 EST-SSR SSR SNP 分子标记 

分 类 号：S567.239[农业科学—中草药栽培]