补阳壮通饮对脑缺血再灌注损伤大鼠的神经保护作用及其可能机制研究  

Neuroprotective Effect of Buyang Zhuangtong Yin on Cerebral Ischemia-reperfusion in Rats and Its Possible Mechanism

作　　者：王亚南 陈真珍[2] 王凯华[1] 黄龙坚 李岩 WANG Yanan;CHEN Zhenzhen;WANG Kaihua(Department of Encephalopathy,Guangxi International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine,Guangxi 530000,China)

机构地区：[1]广西中医药大学附属国际壮医医院脑病科,南宁530201 [2]广西中医药大学,南宁530200 [3]右江民族医学院,百色533000

出　　处：《医学研究杂志》2024年第4期40-45,共6页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81960801,82160946);广西自然科学基金资助项目(2021GXNSFAA196012);广西一流学科建设开放课题(2019XK148);广西壮族自治区中医药管理局自筹经费科研课题(GXZYZ20210103);广西中医药大学第二批“岐黄工程”高层次人才团队培育项目(2021008)。

摘　　要：目的 研究补阳壮通饮对脑缺血再灌注损伤(cerebral ischemia reperfusion injury, CIRI)大鼠的神经保护作用,并基于Nrf2信号通路探讨其作用机制。方法 将SD大鼠分为空白组、假手术组、模型组、补阳壮通饮组、阿司匹林阳性对照组、补阳壮通饮联合阿司匹林组,每日灌胃给药。采用线栓法构建大脑中动脉闭塞再灌注(middle cerebral artery occlusion/reperfusion, MCAO/R)大鼠模型,记录每日神经功能缺损评分,除空白组外,到达第1、3、7天时间节点后,检测大鼠体质量变化率、TTC染色观察脑梗死体积、HE染色观察脑组织病理学改变情况、免疫荧光和Western blot法检测Nrf2及HO-1蛋白表达的情况。结果与模型组比较,补阳壮通饮组大鼠体质量下降程度减轻(P<0.05),给药3天后,ZeaLonga评分显著降低(P<0.05),神经细胞水肿程度减轻,坏死神经细胞减少,补阳壮通饮可以明显上调Nrf2、HO-1蛋白表达(P<0.05),并促进Nrf2核移位。结论 补阳壮通饮可以减轻MCAO/R模型大鼠CIRI后的神经损伤,其作用可能是通过Nrf2/HO-1介导的抗氧化应激通路实现的。Objective To explore the neuroprotective effect of Buyang Zhuangtong Yin on cerebral ischemia-reperfusion injury(CIRI)in rats,and explore its mechanism based on Nrf2signaling pathway.Methods The SD rats were divided into blank group,sham operation group,model group,Buyang Zhuangtong Yin group,aspirin positive control group and Buyang Zhuangtong Yin combined with aspirin group,and administered by gavage daily.The rats model of middle cerebral artery occlusion/reperfusion(MCAO/R)was established by thread occlusion method.The neurological deficit score was recorded every day.Except the blank group,after reaching the time point of 1,3 and 7days,the rate of weight change were measured,the volume of cerebral infarction was observed by TTC staining,the pathological changes of brain tissue were observed by HE staining,and the expression of Nrf2 and HO-1 protein was detected by immunofluorescence and Western blot.Results Compared with the model group,the weight loss of rats in the Buyang Zhuangtong Yin group was reduced(P<0.05),and after 3days of administration,the ZeaLonga score was significantly decreased(P<0.05),the degree of nerve cell edema was reduced,and the necrotic nerve cells was decreased.Buyang Zhuangtong Yin could significantly up-regulated the protein expression of Nrf2 and HO-1(P<0.05)and promote the nuclear translocation of Nrf2.Conclusion Buyang Zhuangtong Yin can alleviate the nerve injury after CIRI in MCAO/R model rats,and its effect may be realized through the anti-oxidative stress pathway mediated by Nrf2/HO-1.

关 键 词：补阳壮通饮 MCAO/R Nrf2通路 氧化应激 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]