初烤阶段酶制剂不同喷施方式下烟叶中挥发性代谢物的差异分析  

Differential Analysis of Volatile Metabolites in Flue-Cured Tobacco Leaves Under Different Spraying Methods of Exogenous Enzyme at Initial Curing Stage

作　　者：郑杰 王平平 郭春生 叶亚军 张傲杰 龚廷锋 艾绥龙 张立新[1] Zheng Jie;Wang Pingping;Guo Chunsheng;Ye Yajun;Zhang Aojie;Gong Tingfeng;Ai Suilong;Zhang Lixin(College of Life Sciences,Northwest Agriculture and Forestry University,Yangling 712100,China;Shaanxi Institute of Tobacco Science,Xi’an 710000,China;Inner Mongolia Kunming Cigarette Co.,Ltd.,Hohhot 010020,China)

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]陕西省烟草科学研究所,陕西西安710000 [3]内蒙古昆明卷烟有限责任公司,内蒙古呼和浩特010020

出　　处：《山东农业科学》2024年第4期56-65,共10页Shandong Agricultural Sciences

基　　金：中国烟草总公司陕西省公司重大科技项目“陕西烟叶质量特色挖掘及彰显技术研究与应用”(SXYC-2021-KJ-01)。

摘　　要：为明确初烤阶段(变黄期)外源酶制剂不同喷施方式下烤后烟叶中挥发性代谢物的差异变化规律,本试验以陕西烟叶主要产区商洛市洛南县的云烟99(B2F)为供试材料,运用液相色谱-质谱(LC-MS)联用技术进行非靶向代谢组学分析,研究酶制剂不同喷施方式(LN和LN-A)和对照(LN-CK)烟叶中挥发性代谢物的差异。结果表明,外源酶制剂喷施方式对烟叶中挥发性代谢物的相对含量产生显著影响,从各样品烟叶中共检测出578种挥发性代谢物,其数量由多到少依次为萜类(99种)、酯类(91种)、杂环化合物(88种)、烃类(63种)、酮类(52种)、醛类(42种)、有机酸(21种)等。PCA和OPLS-DA分析结果显示,LN-CK与LN、LN-CK与LN-A、LN与LN-A之间的代谢物表现出良好分离,分别存在177、121种和175种显著差异代谢物,其中,LN与LN-CK相比,上升的有142种,下降的有35种;LN-A与对照相比,上升的有71种,下降的有50种;LN-A与LN相比有56种上升,119种下降。基于样本间的S-Plot图和火山图,从LN-CK与LN、LN-CK与LN-A、LN与LN-A三组各筛选出20个相对含量差异显著的挥发性物质成分,可用于比较两种酶制剂喷施方式的不同效应,可为外源酶制剂喷施方式的选择提供技术支撑。总起来看,LN这种外源酶制剂喷施方式能更好地正向影响挥发性代谢物种类进而提升烟叶香气品质。In order to clarify the variation rules of differential volatile metabolites in flue-cured tobacco leaves under different spraying methods of exogenous enzyme preparations at the initial curing stage(yelting stage),Yunyan 99(B2F)from Luonan County,Shangluo City,Shaanxi Province was used as test material in this experiment,non-targeted metabolomics analysis was performed by liquid chromatography-mass spectrometry(LC-MS),and the differences in volatile metabolites of flue-cured tobacco leaves under different enzyme spraying methods(LN and LN-A)and the control(LN-CK)were studied.The results showed that the application methods of exogenous enzyme had significant influences on the relative content of volatile metabolites in tobacco leaves.A total of 578 volatile metabolites were detected from tobacco leaves,including terpenoids(99 kinds),esters(91 kinds),heterocyclic compounds(88 kinds),hydrocarbons(63 kinds),ketones(52 kinds),aldehydes(42 kinds),organic acids(21 kinds)and so on.The results of PCA and OPLS-DA analysis showed that the metabolites between LN-CK and LN,LN-CK and LN-A,and LN and LN-A separated well,and 177,121 and 175 kinds significantly different metabolites were observed,respectively.Among which,142 kinds showed increase in relative content and 35 kinds showed decrease in LN treatment compared with LN-CK;71 kinds showed increase and 50 kinds showed decrease in LN-A treatment compared with LN-CK;56 kinds showed increase and 119 kinds showed decrease in LN-A treatment compared with LN treatment.Based on the S-plot and volcano plot between samples,20 volatile components with significant differences in relative content were screened out from each group of LN-CK and LN,LN-CK and LN-A,and LN and LN-A,so that the different effects of the two enzyme spraying treatments could be compared with them.It could provide technical support for the selection of exogenous enzyme spraying methods.All in all,the spraying method of exogenous enzyme preparations in LN treatment could better positively affect the types of vola

关 键 词：烤烟 外源酶制剂 喷施方式 代谢组学 挥发性代谢物 差异分析 

分 类 号：S572.09[农业科学—烟草工业]