直肠癌患者的血清C型凝集素受体及其下游效应分子CARD9表达与临床病理特征和预后的关系  

Relationship between expression of serum C-type lectin receptor and its downstream effector CARD9 and clinicopathological features and prognosis of rectal cancer patients

作　　者：霍成 张孝堂 杨斌健 HUO Cheng;ZHANG Xiaotang;YANG Binjian(Department of Pathology,Sinopharm Tongmei General Hospital,Datong 037003,China;Department of General Surgery,Sinopharm Tongmei General Hospital,Datong 037003,China;Department of Central Laboratory,Sinopharm Tongmei General Hospital,Datong 037003,China)

机构地区：[1]国药同煤总医院病理科,山西大同037003 [2]国药同煤总医院普外科,山西大同037003 [3]国药同煤总医院中心实验室,山西大同037003

出　　处：《国际消化病杂志》2024年第2期94-99,137,共7页International Journal of Digestive Diseases

摘　　要：目的探究直肠癌患者的血清C型凝集素受体(CLR)及其下游效应分子胱天蛋白酶募集域蛋白9(CARD9)表达与临床病理特征和预后的关系。方法选择2018年3月至2020年3月国药同煤总医院收治的98例直肠癌患者作为研究对象(设为观察组),另选择45名同期在该院体检的健康志愿者设为对照组。采用ELISA法检测血清可溶性树突状细胞特异性细胞间黏附分子3结合非整合素(sDC-SIGN)和CARD9表达水平,并分析其与直肠癌患者临床病理特征的关系。采用Pearson相关性分析探讨血清sDC-SIGN与CARD9表达的关系。入组直肠癌患者术后均随访3年,计算3年总生存(OS)率。采用Kaplan-Meier法绘制生存曲线,分析血清sDC-SIGN和CARD9表达与直肠癌患者预后的关系。采用多因素Cox回归分析探讨直肠癌患者预后的影响因素。采用ROC曲线分析血清sDC-SIGN和CARD9对直肠癌患者术后生存情况的预测价值。结果与对照组相比,观察组的血清sDC-SIGN表达水平降低,血清CARD9表达水平升高,2组的差异均有统计学意义(P均<0.05)。Pearson相关性分析结果显示,直肠癌患者的血清sDC-SIGN与CARD9表达呈显著负相关(r=-0.743,P<0.05)。与无淋巴结转移的直肠癌患者相比,有淋巴结转移的患者的血清sDC-SIGN表达水平显著降低,而血清CARD9表达水平显著升高,差异均有统计学意义(P均<0.05)。sDC-SIGN低表达组的3年OS率显著低于sDC-SIGN高表达组,CARD9高表达组的3年OS率显著低于CARD9低表达组,差异均有统计学意义(P均<0.05)。多因素Cox回归分析结果显示,分化程度、TNM分期、淋巴结转移、血清sDC-SIGN和CARD9均是影响直肠癌患者预后的独立危险因素(P均<0.05)。血清sDC-SIGN和CARD9联合预测直肠癌患者术后3年生存情况的ROC曲线下面积(AUC)为0.896,均大于两者单独预测的AUC(P均<0.05)。结论血清sDC-SIGN和CARD9表达水平均与直肠癌发生密切相关,且血清sDC-SIGN低表达及血清CARD9�Objective An attempt is made in this paper to investigate the relationship between the expression of serum C-type lectin receptor(CLR)and its downstream effector caspase recruitment domain protein 9(CARD9)and the clinicopathological features and prognosis of rectal cancer patients.Methods A total of 98 patients with rectal cancer at the Sinopharm Tongmei General Hospital from March 2018 to March 2020 were selected as the subjects(assigned to the observation group),and 45 healthy volunteers who underwent physical examinations at the hospital during the same period were selected and assigned to the control group.The expression of serum soluble dendritic cell-specific intercellular adhesion molecular 3-grabbing nonintegrin(sDC-SIGN)and CARD9 was detected by using the ELISA method,and the relationship between the expression of serum sDC-SIGN and CARD9 and clinicopathological features of rectal cancer patients was analyzed.Pearson correlation analysis was used to explore the relationship between serum sDC-SIGN and CARD9.All enrolled rectal cancer patients were followed up for a period of 3 years after surgery,and the 3-year overall survival(OS)rate was calculated.The Kaplan-Meier method was used to draw survival curves and analyze the relationship between serum sDC-SIGN and CARD9 expression and prognosis of rectal cancer patients.The prognostic factors of rectal cancer patients were explored using multivariate Cox regression analysis.The predictive value of serum sDC-SIGN and CARD9 in postoperative survival of rectal cancer patients was analyzed using ROC curve analysis.Results Compared with the control group,the expression level of serum sDC-SIGN is decreased and the expression level of CARD9 is increased in the observation group,with statistically significant differences(P<0.05).The Pearson correlation analysis shows that there is a significant negative correlation between serum sDC-SIGN and CARD9 expression in rectal cancer patients(r=-0.743 and P<0.05).Compared with rectal cancer patients without lymph node metast

关 键 词：直肠癌 可溶性树突状细胞特异性细胞间黏附分子3结合非整合素 胱天蛋白酶募集域蛋白9 临床病理特征 预后 

分 类 号：R735.37[医药卫生—肿瘤]