结直肠癌患者的血清长链非编码RNASNHG5和SNHG11表达及临床意义  

Expression of clinical significance of lncRNA SNHG5 and SNHG11 in serum of colorectal cancer patients

作　　者：牛志新 何峰 宋春光[1] 肖玉清 NIU Zhixin;HE Feng;SONG Chunguang;XIAO Yuqing(Department of Proctology,the First Hospital of Qinhuangdao,Qinhuangdao 066500,China;Department of the Second External Medicine,Qinglong Manchu Autonomous County Hospital,Qinhuangdao 066500,China)

机构地区：[1]秦皇岛市第一医院肛肠科,066500 [2]秦皇岛市青龙满族自治县医院外二科,066500

出　　处：《国际消化病杂志》2024年第2期100-105,共6页International Journal of Digestive Diseases

摘　　要：目的探究结直肠癌(CRC)患者的血清长链非编码RNA小核仁RNA宿主基因5(lncRNASNHG5,下文简称SNHG5)、SNHG11表达水平及其对CRC的诊断价值。方法选择2015年1月至2018年3月于秦皇岛市第一医院接受CRC根治术治疗的72例CRC患者作为研究对象(设为CRC组),另选择同期于该院就诊的61例结直肠息肉患者设为结直肠息肉组,以及同期于该院体检的59名健康志愿者设为健康对照组。采用实时荧光定量PCR法检测血清SNHG5、SNHG11表达水平,并分析其与CRC患者临床病理特征的关系。采用ROC曲线评估血清SNHG5、SNHG11诊断CRC的效能。采用Kaplan-Meier法分析血清SNHG5、SNHG11表达水平与CRC患者术后无病生存期的关系。结果与健康对照组相比,结直肠息肉组和CRC组的血清SNHG5、SNHG11相对表达量均较高,且CRC组的血清SNHG5、SNHG11相对表达量均高于结直肠息肉组,差异均有统计学意义(P均<0.05)。低分化、TNM分期为Ⅲ期、有浆膜浸润、有淋巴结转移的CRC患者的血清SNHG5、SNHG11相对表达量分别高于中高分化、TNM分期为Ⅱ期、无浆膜浸润、无淋巴结转移的患者,差异均有统计学意义(P均<0.05)。ROC曲线分析结果显示,血清SNHG5和SNHG11联合检测诊断CRC的AUC均大于单项检测,其敏感度和特异度也均高于单项检测。SNHG5低表达组和SNHG11低表达组的无病生存率分别高于SNHG5高表达组和SNHG11高表达组,差异均有统计学意义(P均<0.05)。结论CRC患者的血清SNHG5、SNHG11表达均上调,且均与患者术后无病生存密切相关,两者联合检测诊断CRC的效能较高。Objective This paper attempts to explore the expression of long non-coding RNA small nucleolar RNA host gene 5(lncRNA SNHG5,hereinafter referred to as SNHG5)and SNHG11 in serum of colorectal cancer patients and their diagnosis value for colorectal cancer.Methods A total of 72 CRC patients treated with CRC radical resection at the First Hospital of Qinhuangdao from January 2015 to March 2018 were selected as the study subjects(assigned to the CRC group)while 61 patients with colorectal polyps treated in the same hospital in the same period were selected as the colorectal polyp group,and 59 healthy volunteers who underwent physical examinations in the same hospital were selected as the healthy control group.The expression of serum SNHG5 and SNHG11 was detected by real-time quantitative PCR,and the relationship between the expression of serum SNHG5 and SNHG11 and clinicopathological features of CRC was analyzed.The ROC curve was used to evaluate the efficacy of serum SNHG5 and SNHG11 in the diagnosis of CRC.The relationship between the expression of serum SNHG5 and SNHG11 and disease-free survival was analyzed by using the Kaplan-Meier method.Results Compared with the healthy control group,the relative expression levels of serum SNHG5 and SNHG11 in the colorectal polyp group and the CRC group are higher,and the relative expression levels of serum SNHG5 and SNHG11 in the CRC group are higher than those in the colorectal polyp group,with statistically significant differences(P<0.05).The relative expression levels of serum SNHG5 and SNHG11 of CRC patients with low differentiation,stageⅢ,serosal invasion and lymph node metastasis are higher than those of CRC patients with medium or high differentiation,stageⅡ,no serosal invasion and lymph node metastasis,respectively,with statistically significant differences(P<0.05).The ROC curve analysis shows that the AUC of serum SNHG5 and SNHG11 is higher than that of single detection,and the sensitivity and specificity of serum SNHG5 and SNHG11 are higher than those of single

关 键 词：结直肠癌 长链非编码RNA 小核仁RNA宿主基因5 小核仁RNA宿主基因11 诊断 预后 

分 类 号：R735.34[医药卫生—肿瘤]