‘威代尔’葡萄及其优良芽变果实香气物质2-苯乙醇合成变化研究  

Study on the Synthetic Change of Aroma 2-phenylethanol in‘Vidal’Grape and Its Bud Sport

作　　者：林玉友 郭修武[2] 蒋春光 郭印山[2] 申海林[3] 郑娟 LIN Yuyou;GUO Xiuwu;JIANG Chunguang;GUO Yinshan;SHEN Hailin;ZHENG Juan(Institute of Agriculture and Forestry in Arid Areas of Liaoning Province,Chaoyang,Liaoning 122000;College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110866;Institute of Pomology,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100)

机构地区：[1]辽宁省旱地农林研究所,辽宁朝阳122000 [2]沈阳农业大学园艺学院,辽宁沈阳110866 [3]吉林省农业科学院果树研究所,吉林公主岭136100

出　　处：《北方园艺》2024年第8期27-33,共7页Northern Horticulture

基　　金：国家现代葡萄产业技术体系岗位专家资助项目(CARS-29-yc-6)。

摘　　要：以‘威代尔’葡萄及其芽变果实为试材,采用氨基酸分析仪和荧光定量分析,研究了‘威代尔’葡萄及其芽变果实中的苯丙氨酸、苯乙胺、苯乙醛和2-苯乙醇动态含量,以及芳香族氨基酸脱羧酶(AADC)的动态相对表达量,以期明确‘威代尔’葡萄优良芽变果实特征香气2-苯乙醇合成变化,为生产提供优良酿造品种(系)原料。结果表明:‘威代尔’葡萄及其芽变果实中不同生育期苯丙氨酸、苯乙胺、苯乙醛和2-苯乙醇含量均呈现规律性变化,芽变果实中苯丙氨酸、苯乙胺、苯乙醛和2-苯乙醇含量均高于同期‘威代尔’果实。荧光定量结果表明,‘威代尔’及其果实中AADC的表达与2-苯乙醇含量均呈正相关。该研究发现的‘威代尔’葡萄芽变有望成为我国酿造优良葡萄酒重要的品种(系)原料。Taking the‘Vidal'grape and its bud variant fruit as the test materials,and amino acid analyzer and fluorescence quantitative analysis were used,the dynamic content of phenylalanine,phenylethylamine,phenylacetaldehyde,and 2-phenylethanol in the‘Vidal'grape and its bud variant fruit were studied,as well as the dynamic relative expression of AADC enzyme,in order to clarify the synthesis changes the characteristic aroma of 2-phenylethanol in the excellent bud variant of the‘Vidal'grape,and the source were provided with raw materials for the production of excellent brewing varieties(series).The results showed that the content of phenylalanine,phenylacetamine,phenylacetaldehyde and 2-phenylethanol of the fruit was changed regularly in‘Vidal'grape and bud variant,and the content of phenylalanine,phenacetamine,phenylacetaldehyde and 2-phenylethanol in the‘Vidal'grape were higher than bud variant.The fluorescence quantification showed that AADC enzyme expression was positively correlated with 2-phenylethanol content in both‘Vidal'and bud variant.The bud variant of‘Vidal'was expected to provide an important variety(series)for the production of excellent wine in China.

关 键 词：‘威代尔’ 葡萄 芽变 2-苯乙醇 芳香族氨基酸脱羧酶 

分 类 号：S663.1[农业科学—果树学]