基于白尾海雕粪便样品的SSR引物及性别鉴定引物的开发  

Microsatellite and sex identification primers for the white-tailed sea eagle

作　　者：袁嘉瞳 牛文会 张轶卓 贾婷 何绍纯[2] 鲍蕾[1] YUAN Jiatong;NIU Wenhui;ZHANG Yizhuo;JIA Ting;HE Shaochun;BAO Lei(Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering,National Forestry and Grassland Administration Key Laboratory for Conservation Ecology in the Northeast Tiger and Leopard National Park,College of Life Sciences,Beijing Normal University,Beijing,China;Beijing Zoo,Beijing,China;Beijing Key Laboratory of Captive Wildlife Technologies,Beijing,China)

机构地区：[1]生物多样性与生态工程教育部重点实验室,东北虎豹国家公园保护生态学国家林草局重点实验室,北京师范大学生命科学学院,北京 [2]北京动物园管理处,北京 [3]圈养野生动物技术北京市重点实验室,北京

出　　处：《北京师范大学学报（自然科学版）》2024年第2期190-194,共5页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金面上资助项目(32171654)。

摘　　要：白尾海雕(Haliaeetus albicilla),是鹰形目鹰科海雕属的一种大型猛禽、国家一级保护动物,位于食物链顶端,对于维持生态平衡和生物多样性具有重要作用.每年冬季,大批白尾海雕在我国吉林省珲春市敬信湿地越冬,形成了国内最大的越冬种群.为了实现基于粪便样品开展白尾海雕种群遗传学等方面的研究的可行性,填补白尾海雕亚洲种群的研究空白,需要开发新的微卫星DNA,即SSR(simple sequence repeat)引物和性别鉴定引物.本研究以NCBI上公布的白尾海雕全基因组数据为基础,进行了SSR引物和性别鉴定引物的开发.随后,使用白尾海雕粪便样品对引物进行了筛选,最终获得9对SSR引物和2对性别鉴定引物.本研究为白尾海雕种群遗传学深入研究提供了丰富有效的SSR分子标记,也为白尾海雕性别分子鉴定提供了新的引物选择.White-tailed sea eagle(Haliaeetus albicilla),a large raptor belonging to Accipitridae of Accipitriformes,is a first-class national protected animal in China.This top predator plays a significant role in maintaining ecosystem balance and biodiversity.During the winter,a large wintering population is present in Jingxin Wetland in Hunchun city,Jilin province.To study the population genetics of white-tailed sea eagles,it is necessary to develop microsatellite SSR(simple sequence repeat)and sex identification primers.In this study,microsatellite and sex identification primers were designed based on the whole genome data of white-tailed sea eagle(NCBI),and verified with white-tailed sea eagle fecal samples collected from Jingxin Wetland.A total of 9 pairs of SSR primers and 2 pairs of sex identification primers were confirmed,which will be useful for future studies of the white-tailed sea eagle.

关 键 词：SSR分子标记 性别鉴定 引物开发 粪便样品 

分 类 号：Q951[生物学—动物学]