基于四氧化三铁/聚(苯乙烯-4-乙烯吡啶)的食品中四环素残留的快速筛选  

Rapid screening of tetracycline residues in food based on Fe_(3)O_(4)/poly(styrene-4-vinyl pyridine)nanocomposites

作　　者：沈唐文 折欢欢 张毅 严恒 谢卫红 SHEN Tangwen;SHE Huanhuan;ZHANG Yi;YAN Heng;XIE Weihong(College of Biotechnology and Food,Hubei University of Technology,Wuhan 430068,China;Hubei Provincial Institute for Food Supervision and Test,Wuhan 430075,China)

机构地区：[1]湖北工业大学生物工程与食品学院,武汉430068 [2]湖北省食品质量安全监督检验研究院,武汉430075

出　　处：《分析试验室》2024年第4期511-516,共6页Chinese Journal of Analysis Laboratory

基　　金：湖北省食品质量安全监督检验研究院科技项目(ZZLX2015015);湖北工业大学“产品质量工程”跨学科研究院开放课题(00021)资助。

摘　　要：基于四环素对四氧化三铁(Fe_(3)O_(4))/聚(苯乙烯-4-乙烯吡啶)(Fe_(3)O_(4)/P(st-4vp))的过氧化物酶活性的抑制作用,建立了一种快速筛选牛奶和蜂蜜中四环素残留的方法。酸性条件下,所制备的Fe_(3)O_(4)/P(st-4vp)能够催化H_(2)O_(2)氧化3,3,5,5’-四甲基联苯胺(TMB)生成蓝色的氧化TMB(ox TMB),在652 nm处出现可见吸收特征峰。四环素对该反应产生抑制作用,在p H 5.0、50℃水浴、反应时间20 min、H_(2)O_(2)浓度10 mmol/L、Fe_(3)O_(4)/P(st-4vp)浓度1.0 mg/mL、TMB浓度0.36 mmol/L条件下,四环素对Fe_(3)O_(4)/P(st-4vp)酶活性的抑制作用最强。根据四环素浓度与酶抑制率的关系曲线,对牛奶和蜂蜜中的加标四环素进行了筛查。结果表明,Fe_(3)O_(4)/P(st-4vp)对四环素加标量范围5~197.5μg/kg的牛奶和蜂蜜的筛查准确度分别为94.4%和96.3%。A rapid screening method for tetracycline residues in milk and honey samples was developed based on the inhibitory effect of tetracycline on the peroxidase activity of Fe_(3)O_(4)/poly(styrene-4-vinylpyridine)(Fe_(3)O_(4)/P(st4vp)).Under acidic conditions,the prepared Fe_(3)O_(4)/P(st-4vp)was able to catalyze the oxidation of 3,3,5,5′-tetramethylbenzidine(TMB)by H_(2)O_(2) to produce blue oxidized TMB(oxTMB),with a characteristic absorption peak at 652 nm.The reaction was inhibited by tetracycline,which showed the strongest inhibition of Fe_(3)O_(4)/P(st4vp)enzyme activity under conditions of pH 5.0,50℃of water bath,20 min of reaction time,10 mmol/L of H_(2)O_(2),1.0 mg/mL of Fe_(3)O_(4)/P(st-4vp),and 0.36 mmol/L of TMB.Based on the relationship curve between tetracycline concentration and enzyme inhibition rate,the spiked tetracycline in milk and honey samples was screened,and the results showed that the screening range of Fe_(3)O_(4)/P(st-4vp)for tetracycline spiked in milk and honey was 5-197.5µg/kg,and the relative accuracy were 94.4%and 96.3%,respectively.

关 键 词：四氧化三铁 聚(苯乙烯-4-乙烯吡啶) 纳米酶 四环素残留 快检 

分 类 号：TS207.3[轻工技术与工程—食品科学]