Molecular docking insights into nuclear factor Y(NF-Y)transcription factor and pyrabactin resistance 1(PYL)receptor proteins reveal abiotic stress regulation in finger millet  

作　　者：Varsha Rani Vinay Kumar Singh D.C.Joshi Rajesh Singh Dinesh Yadav 

机构地区：[1]Department of Biotechnology,Deen Dayal Upadhyaya Gorakhpur University,Gorakhpur,273009,Uttar Pradesh,India [2]Centre for Bioinformatics,School of Biotechnology,Banaras Hindu University,Varanasi,221005,Uttar Pradesh,India [3]ICAR-Vivekananda Institute of Hill Agriculture,Almora,263601,Uttarakhand,India [4]Department of Genetics and Plant Breeding,Institute of Agricultural Sciences,Banaras Hindu University,Varanasi,Uttar Pradesh,221005,India

出　　处：《Crop Design》2024年第1期35-44,共10页作物设计（英文）

摘　　要：Finger Millet(Eleusine coracana-(L.)Gaertn),is an important nutraceutical crop with the potential for imparting food and nutritional security.These plants have a comparatively higher tolerance for several abiotic stresses like drought,salinity,and heat.Several players including Transcription Factor(TF)like Nuclear Factor Y(NF-Y)might be associated with this enhanced level of tolerance.Further,it is unclear how phytohormones like Abscisic acid(ABA)regulate the expression of NF-Y,whether in ABA-dependent or ABA-Independent pathway.The interaction of PYL(Pyrabactin resistance1-like)receptor proteins with Nuclear Factor Y(NF-Y)Transcription Factor in the presence of phytohormones like abscisic acid(ABA)provides one insight related to the enhanced tolerance towards abiotic stresses under ABA-dependent signaling in finger millet crop.A total of three PYL receptors of finger millet designated as EcPYL1,EcPYL5,and EcPYL9 were retrieved in the finger millet genome.These receptors were modeled through the SWISS-MODEL using templates 5gwo and 3wg8 and docked with ABA.The bestdocked protein-ligand complex PYL5-ABA(binding energyΔG=-8.8 kcal mol^(-1))was found to be most stable at the 50ns MD simulation study.Further protein-protein interaction between PYL5 and NF-YA2/B3/C1 subfamily members showed a good interaction.This clearly indicates the possibility of the NF-Y-PYL module in the ABA transduction pathway,which performs a crucial role in the expression of stress-responsive genes.These studies reveal the intricate relationship between the ABA,PYL receptors of finger millet,and NF-Y transcription factor in regulating the stress-responsive genes and provide an insight into the abiotic stress tolerance mechanisms,which can be targeted for crop improvement.

关 键 词：ABA receptors PYL NF-Y molecular docking Abiotic stress MD simulation Finger millet HOTSPOT 

分 类 号：S515[农业科学—作物学] Q945.78[生物学—植物学]