新疆哈萨克族H型高血压与MTHFR基因多态性及叶酸水平的相关性研究  

Association of MTHFR Gene Polymorphisms and Folate with H-Type Hypertension in Xinjiang Kazakhs

作　　者：廖晟宇 马儒林[1] 张向辉[1] 王馨平 何欣 郭恒[1] LIAO Sheng-yu;MA Ru-lin;ZHANG Xiang-hui;WANG Xin-ping;HE Xin;GUO Heng(Department of Preventive Medicine,Shihezi University School of Medicine,Xinjiang Shihezi,832002)

机构地区：[1]石河子大学医学院预防医学系,新疆石河子832002

出　　处：《农垦医学》2024年第1期20-25,77,共7页Journal of Nongken Medicine

基　　金：新疆生产建设兵团财政科技计划项目(2021AB030);石河子大学创新发展项目(CXFZ202005)。

摘　　要：目的:探讨哈萨克族H型高血压与同型半胱氨酸(Hcy)代谢相关基因MTHFR多态性和叶酸的关联,为H型高血压发病机制提供理论依据。方法:应用新疆哈萨克族H型高血压现况调查数据库,采用病例-对照研究,随机选取H型高血压患者100例、单纯高血压患者100例、高Hcy血症患者100例,健康人群100例作为对照组。应用Sanger测序法对所有研究对象的MTHFR基因rs1801133、rs1801131位点进行基因型检测。叶酸采用电化学发光法进行检测。采用χ^(2)检验进行Hardy-Weinberg平衡检验;应用二分类非条件Logistic回归分析H型高血压与MTHFR基因位点以及叶酸之间的关联。结果:以健康组为对照,高水平叶酸是哈萨克族H型高血压的保护因素(OR=0.33,95%CI:0.15-0.72),rs1801133-(CT+TT)基因型是危险因素(OR=6.37,95%CI:2.97-13.68);高水平叶酸是哈萨克族高Hcy血症的保护因素(OR=0.45,95%CI:0.23-0.86),rs1801133-(CT+TT)基因型是危险因素(OR=2.92,95%CI:1.52-5.62)。结论:MTHFR基因的rs1801133-T等位基因和rs1801133-(CT+TT)基因型是H型高血压的危险因素;高水平叶酸是H型高血压的保护因素。Objective:To explore the associations of homocysteine(Hcy)metabolism-related genes MTHFR polymorphisms and folate with H-type hypertension in Kazakhs.These results provide support and a theoretical basis for the pathogenesis research of H-type hypertension.Methods:A case-control study,using data from the"Xinjiang Kazakhs H-type ypertension Cross-sectional study"was conducted.A total of 400 subjects were randomly selected,which included 300 cases and 100 controls.Among them,the case groups included 100 patients with H-type hypertension,100 patients with uncomplicated hypertension,and 100 patients with Hyperhomocysteinemia(HHcy).Genetic testing of MTHFR(rs1801133,rs1801131)was performed using Sanger sequencing.Folate was detected by electrochemiluminescence assay.Hardy-Weinberg equilibrium was tested by Pearson's chi-square(χ^(2))test.We applied unconditional logistic regression to analysis was carried out to analyze the association of MTHFR gene and folate with H-type hypertension.Results:Compared with the healthy control group,the high level of folate was a protective factor for H-type hypertension(OR=0.33,95%CI:0.15-0.72),while rs1801133-(CT+TT)was a risk factor(OR=6.37,95%CI:2.97-13.68).Meanwhile,the high level of folate was a protective factor for HHcy(OR=0.45,95%CI:0.23-0.86),while rs1801133-(CT+TT)was a risk factor(OR=2.92,95%CI:1.52-5.62).Conclusions:The T allele and CT+TT genotypes of MTHFR rs1801133 were risk factors for H-type hypertension in Kazakhs,while the high level of folate was a protective factor for H-type hypertension.

关 键 词：哈萨克族 H型高血压 MTHFR基因 叶酸 

分 类 号：R783.6[医药卫生—口腔医学]