基于PS信号放大策略的SERS适配体传感器灵敏检测恩诺沙星  

A SERS Aptasensor Based on PS⁃Mediated Signal Amplification Strategy for the Sensitive Detection of Enrofloxacin

作　　者：李岑 王盼雪 王丽 刘莹 邢招娣 李国梁 LI Cen;WANG Panxue;WANG Li;LIU Ying;XING Zhaodi;LI Guoliang(School of Food Science and Engineering,Shaanxi University of Science and Technology,Xi′an 710021,Shaanxi,China;Pini Testing Group Shaanxi Co.,Ltd.,Xi′an 710018,Shaanxi,China)

机构地区：[1]陕西科技大学食品科学与工程学院,陕西西安710021 [2]谱尼测试集团陕西有限公司,陕西西安710018

出　　处：《食品研究与开发》2024年第9期150-158,共9页Food Research and Development

基　　金：国家自然科学基金项目(32102063);陕西科技大学高水平博士人才科研启动项目(2017BJ-51);大学生创新创业训练计划项目(S20210708054)。

摘　　要：利用表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)技术检测动物源食品中的抗生素残留操作简便、用时短,但易受基质干扰、灵敏度偏低。该研究利用SERS技术结合磁分离和信号放大策略开发一种高灵敏SERS适配体传感器,用于恩诺沙星的快速检测。首先,利用聚苯乙烯微球(polystyrene microspheres,PS)、4-MBA和互补DNA(complementary DNA,cDNA)构建信号放大型拉曼探针4-MBA@PS@cDNA,利用适配体(aptamer,Apt)和磁珠(magnetic bead,MB)构建捕获探针MB@Apt,并通过碱基互补配对将4-MBA@PS@cDNA和MB@Apt组装成复合探针。结果表明,4-MBA位于1 078 cm^(-1)处的SERS信号强度与恩诺沙星浓度的对数值在1~100 nmol/L具有良好的线性相关关系,决定系数为0.956 6。该方法对恩诺沙星的检出限为0.71 nmol/L,检测牛奶和猪肉中恩诺沙星的回收率为86.67%~128.00%,相对标准偏差为0.4%~1.3%。It is simple and time⁃efficient to determine the antibiotic residues in animal⁃derived food using sur⁃face enhanced Raman spectroscopy(SERS),but the technology is susceptible to matrix interference and ex⁃hibited low sensitivity.In this paper,a highly⁃sensitive SERS aptasensor for the rapid detection of enrofloxacin was developed by SERS combined with magnetic separation and signal amplification strategy.First of all,the Raman probe 4⁃MBA@PS@cDNA with signal amplification function was constructed using polystyrene micro⁃spheres(PS),4⁃MBA and complementary DNA(cDNA).The capture probe MB@Apt was constructed using aptamer(Apt)and magnetic bead(MB).The 4⁃MBA@PS@cDNA and MB@Apt were assembled into a com⁃posite probe by base complementary pairing.The results showed that there was a good linear relationship be⁃tween the SERS signal intensity of 4⁃MBA at the Raman shift of 1078 cm−1 and the logarithm value of the enro⁃floxacin concentration in the range of 1−100 nmol/L,and the coefficient of determination was 0.9566.The de⁃tection limit of enrofloxacin was 0.71 nmol/L,the recovery of enrofloxacin in milk and pork was between 86.67%and 128.00%,and the relative standard deviation varied between 0.4%and 1.3%.

关 键 词：恩诺沙星 快速检测 表面增强拉曼光谱 聚苯乙烯微球 适配体 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.37[轻工技术与工程—食品科学与工程]