肝细胞癌组织BCLAF1和TCF3 RNA水平及其临床意义探讨  

Increased cancerous tissue BCLAF1 and TCF3 levels is correlated to poor prognosis in patients with hepatocellular carcinoma

作　　者：倪裕丰 倪国英 杨志勇 Ni Yufeng;Ni Guoying;Yang Zhiyong(Department of Oncology,Chongming Hospital Affiliated to Shanghai Medical College of Health,Shanghai 202150,China)

机构地区：[1]上海健康医学院附属崇明医院肿瘤内科,上海市202150

出　　处：《实用肝脏病杂志》2024年第3期418-421,共4页Journal of Practical Hepatology

基　　金：上海市卫生健康委员会科研项目(编号:201940274)。

摘　　要：目的研究肝细胞癌(HCC)患者癌组织Bcl-2相关转录因子1(BCLAF1)和T细胞因子3(TCF3)RNA水平变化及其临床意义。方法2017年1月~2022年1月我院诊治的62例HCC患者,均接受肝叶切除术治疗,取得癌组织和癌旁肝组织。术后,随访2年。常规行病理学检查,采用qRT-PCR法检测组织BCLAF1 RNA和TCF3 RNA水平。结果HCC患者癌组织BCLAF1 RNA和TCF3 RNA水平分别为(1.5±0.2)和(1.9±0.3),显著高于癌旁组织【分别为(0.9±0.1)和(1.2±0.1),P<0.05】;直径>3 mm癌组织BCLAF1 RNA水平为(1.7±0.3),显著高于直径≤3 mm肿瘤【(1.3±0.2,P<0.05】;有包膜侵犯的癌组织BCLAF1 RNA水平为(1.6±0.3),显著高于无包膜侵犯肿瘤【(1.4±0.2,P<0.05】;中/低分化肿瘤BCLAF1 RNA和TCF3 RNA水平显著高于高分化肿瘤(P<0.05);TNM分期Ⅰ期和Ⅱ期肿瘤BCLAF1 RNA和TCF3 RNA水平显著低于Ⅲ期肿瘤(P<0.05);有微血管侵犯肿瘤BCLAF1 RNA和TCF3 RNA水平分别为(1.6±0.3)和(2.0±0.3),显著高于无微血管侵犯肿瘤【分别为(1.3±0.2)和(1.7±0.3),P<0.05】;有淋巴结转移肿瘤BCLAF1 RNA和TCF3 RNA水平分别为(1.6±0.2)和(2.1±0.4),显著高于无淋巴结转移肿瘤【分别为(1.4±0.2)和(1.7±0.3),P<0.05】;随访2年,62例HCC患者生存38例(61.3%);死亡患者癌组织BCLAF1 RNA和TCF3 RNA水平分别为(1.7±0.3)和(2.2±0.4),显著高于生存组【分别为(1.4±0.2)和(1.7±0.3),P<0.05】。结论HCC患者癌组织BCLAF1和TCF3 RNA水平均呈上调趋势,并与肿瘤的恶性程度和不良预后相关,值得进一步研究。Objective The aim of this study was to explore the clinical implication of Bcl-2-associated transcription factor 1(BCLAF1)and T cell factor 3(TCF3)levels in cancerous tissues in patients with hepatocellular carcinoma(HCC).Methods 62 patients with HCC were enrolled in our hospital between January 2017 and January 2022,and all underwent hepatectomy.The cancerous and its adjacent liver tissues were collected.The pathological examinations were routinely observed,and the BCLAF1 RNA and TCF3 RNA loads in cancerous and adjacent liver tissues were assayed by qRT-PCR.All patients with HCC were followed-up for two years.Results The BCLAF1 RNA and TCF3 RNA loads in cancerous tissues in patients with HCC were(1.5±0.2)and(1.9±0.3),significantly higher than[(0.9±0.1)and(1.2±0.1),respectively,P<0.05]in adjacent liver tissues;the cancerous BCLAF1 RNA level in patients with tumor diameter>3 mm was(1.7±0.3),significantly higher than[(1.3±0.2),P<0.05]in patients with tumor diameter≤3 mm;the cancerous BCLAF1 RNA level in patients with capsular invasion was significantly higher than that in patients without capsular invasion[(1.6±0.3)vs.(1.4±0.2),P<0.05];the cancerous BCLAF1 RNA and TCF3 RNA levels in patients with moderate/low differentiation were much higher than in patients with high tumor cell differentiation(P<0.05),and the cancerous BCLAF1 RNA and TCF3 RNA levels in patients with TNM stageⅠandⅡwere significantly lower than in patients with stageⅢ(P<0.05);the cancerous BCLAF1 RNA and TCF3 RNA in patients with microvascular invasion were(1.6±0.3)and(2.0±0.3),significantly higher than[(1.3±0.2)and(1.7±0.3),P<0.05]in patients without microvascular invasion;the BCLAF1 RNA and TCF3 RNA loads in patients with lymph node metastasis were(1.6±0.2)and(2.1±0.4),significantly higher than[(1.4±0.2)and(1.7±0.3),P<0.05]in patients without lymph node metastasis;38 patients(61.3%)survived at the end of two-year follow-up,and the cancerous BCLAF1 RNA and TCF3 RNA loads in dead group were(1.7±0.3)and(2.2±0.4),much higher

关 键 词：肝细胞癌 Bcl-2相关转录因子1 T细胞因子3 病理学特征 预后 

分 类 号：R735.7[医药卫生—肿瘤]