苦草附生细菌的分离鉴定及高效功能菌株筛选  

Isolation and identification of epiphytic bacteria from Vallisneria natans and screening of functional strains

作　　者：梁淑雅 崔陈陈 胡思文 曾巾 丁程成 崔益斌 赵大勇[1] LIANG Shu-ya;CUI Chen-chen;HU Si-wen;ZENG Jin;DING Cheng-cheng;CUI Yi-bin;ZHAO Da-yong(The National Key Laboratory of Water Disaster Prevention,Hohai University,Nanjing 210098,China;State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,Nanjing 210008,China;Nanjing Institute of Environmental Sciences,Ministry of Ecology and Environment,Nanjing 210042,China)

机构地区：[1]河海大学,水灾害防御全国重点实验室,江苏南京210098 [2]中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室,江苏南京210008 [3]生态环境部南京环境科学研究所,江苏南京210042

出　　处：《中国环境科学》2024年第5期2630-2641,共12页China Environmental Science

基　　金：国家重点研发计划资助项目(2022YFC3204004-003);国家自然科学基金资助项目(31971478,32171563);生态环境部南京环境科学研究所创新团队资助项目(ZX2023QT017);中国科学院南京地理与湖泊研究所青年科学家小组资助项目(2021NIGLAS-CJH01)。

摘　　要：为探究沉水植物附生细菌的功能特性,选取苦草(Vallisneria natans)在室内配置模拟污水进行菌群驯化,分离苦草根、叶际附生细菌进行筛选,结合16S rRNA基因序列分析鉴定结果,共分离纯化和保藏172株菌株(分布于5个门、15个目、42个属),其中根际分离菌107株,叶际分离菌65株.将分离得到的5个门、30个属、47个种的82株代表性菌株进行脱氮、解磷释磷、产吲哚乙酸(IAA)和去除有机物功能验证实验.最终筛选到高效氨氮去除菌10株、硝氮去除菌9株、解无机磷菌12株、解有机磷菌11株、产IAA菌11株及去除有机物菌10株.苦草根、叶际可培养微生物多样性较为丰富,该研究中筛选到的高效功能菌丰富了对苦草附生微生物资源的认识,为沉水植物合成菌群的研制和应用奠定基础.In order to explore the functional characteristics of epiphytic bacteria in submerged macrophytes,Vallisneria natans was selected to domesticate the bacterial community in indoor simulated wastewater,screening the rhizosphere and phyllosphere bacteria isolated from V.natans,combined with the 16S rRNA gene sequence analysis and identification results,172 strains were isolated,purified and preserved(distributed in 5 phyla,15 order and 42 genera),including 107 rhizosphere strains and 65 phyllosphere strains.82 representative strains(distributed in 5 phyla,30 genera and 47 species)were selected for nitrogen removal,phosphorus solubilization,indoleacetic acid production and organic matter removal function validation experiments.Finally,10 high-efficiency ammonia nitrogen removal strains,9 high-efficiency nitrate nitrogen removal strains,12 strains with high-efficiency inorganic phosphorus-solubilizing ability,11 strains with high-efficiency organic phosphorus-solubilizing ability,11 strains of high IAA production and 10 high-efficiency organic matter removal strains were screened.The diversity of cultivable microorganisms in the rhizosphere and phyllosphere of V.natans is relatively abundant,the high-efficiency functional strains screened in this study have enriched our cognizance of the resources of epiphytic microorganisms in V.natans,laying the foundation for the development and application of submerged macrophytes synthetic microbial communities.

关 键 词：苦草 附生细菌 分离鉴定 降解作用 功能菌株 

分 类 号：X172[环境科学与工程—环境科学]