介质阻挡放电协同Ag-Cu/TiO_(2)-CS复合光催化剂灭活生物气溶胶的实验研究  

Experimental study on the inactivation of bioaerosols by dielectric barrier discharge combined with Ag-Cu/TiO_(2)-CS composite photocatalyst

作　　者：赵璐瑶 张佳丽 张轲[1] 李彦鹏[1,2] ZHAO Lu-yao;ZHANG Jia-li;ZHANG Ke;LI Yan-peng(School of Water and Environment,Chang’an University,Xi’an 710054,China;Key Laboratory of Subsurface Hydrology and Ecology in Arid Areas,Ministry of Education,Chang’an University,Xi’an 710054,China)

机构地区：[1]长安大学水利与环境学院,西安710054 [2]长安大学旱区地下水文与生态效应教育部重点实验室,西安710054

出　　处：《中国环境科学》2024年第5期2777-2785,共9页China Environmental Science

基　　金：国家重点研发计划(2023YFC3708203);陕西省自然科学基金(2023-JC-YB-216)。

摘　　要：选用大肠杆菌和杂色曲霉菌作为代表性细菌和真菌以制备生物气溶胶,采用溶胶-凝胶法制备了壳聚糖(CS)修饰的Ag-Cu/TiO_(2)-CS复合光催化剂,使用自制线管式介质阻挡放电反应器协同光催化剂对生物气溶胶进行灭活实验研究,考察初始浓度、放电功率、单一及协同处理对生物气溶胶的灭活影响,并通过SEM、胞外蛋白质与电解质测定探究了生物气溶胶灭活机理.结果表明:Ag、Cu掺杂比为2:2mol%时光催化剂灭菌效果最好,处理时间为10.5s时对大肠杆菌的灭活率为0.61-lg.相比介质阻挡放电单一处理,协同光催化处理后,菌体结构破损加重,胞内物质外泄增多,灭菌效率明显提升,在60W/催化灭活条件下,细菌、真菌的灭活率分别为2.89-lg、2.87-lg,且有效抑制生物气溶胶的复活,灭活后48h内细菌、真菌的复活率分别为-3.40-lg、-3.39-lg.Escherichia coli and Aspergillus niger were selected as model organisms to prepare typical bacterial and fungal aerosols,respectively.A chitosan(CS)-modified Ag-Cu/TiO_(2)-CS composite photocatalyst was synthesized via the sol-gel method.The efficacy of this photocatalyst,in conjunction with a custom-built cylindrical dielectric barrier discharge(DBD)reactor,was assessed for bioaerosol inactivation.The study examined the impact of initial concentration,discharge power,and the synergy between photocatalytic and plasma treatments on bioaerosol deactivation.Mechanistic insights were garnered through SEM analysis,as well as extracellular protein and electrolyte assays.The optimal bactericidal effect was achieved with an Ag and Cu doping ratio of 2:2mol%,resulting in a 0.61-lg reduction in E.coli viability within 10.5seconds.Compared to standalone DBD treatment,the combined approach significantly enhanced bacterial and fungal inactivation rates to 2.89-lg and 2.87-lg,respectively,under a 60W condition,and substantially inhibited bioaerosol revival.Post-inactivation,the revival rates of bacteria and fungi were reduced to-3.40-lg and-3.39-lg,respectively,within 48 hours,underscoring the enhanced efficiency of the synergistic treatment.

关 键 词：生物气溶胶灭活 光催化 介质阻挡放电 协同效应 

分 类 号：X171.5[环境科学与工程—环境科学] X513