A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus  

作　　者：Lingzhai Meng Mengmeng Yu Suyan Wang Yuntong Chen Yuanling Bao Peng Liu Xiaoyan Feng Tana He Ru Guo Tao Zhang Mingxue Hu Changjun Liu Xiaole Qi Kai Li Li Gao Yanping Zhang Hongyu Cui Yulong Gao 

机构地区：[1]Avian Immunosuppressive Diseases Division,State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,ChineseAcademy of Agricultural Sciences,Harbin150069,China Avian ImmunosuppressiveDiseases Division,StateKey LaboratoryforAnimal Disease Cont [2]National Poultry Laboratory Animal Resource Center,Harbin 150069,China

出　　处：《Journal of Integrative Agriculture》2024年第5期1658-1670,共13页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2022YFD1800604);the China Agricultural Research System (CARS-41);the Heilongjiang Touyan Innovation Team Program of China

摘　　要：Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.

关 键 词：avian metapneumovirus ATTENUATED protection vaccine candidate CHICKENS 

分 类 号：S858.3[农业科学—临床兽医学]