Gli2调控Hedgehog通路活化对Tca8113细胞增殖、转移和上皮间充质转化的影响  

Gli2 regulates the activation of Hedgehog pathway in proliferation,metastasis,and epithelial mesenchymal transformation of Tca8113 cells

作　　者：刘茂林[1] 王晓堂 宋晓娜 马云辉 常潇琪 宋国华 LIU Maolin;WANG Xiaotang;SONG Xiaona;MA Yunhui;CHANG Xiaoqi;SONG Gouhua(Asset Management Department,Shanxi Medical University,Taiyuan 030001,China;Shanxi Key Laboratory of Laboratory Animals and Animal Models of Human Disease,Center for Laboratory Animals,Shanxi Medical University,Taiyuan 030001)

机构地区：[1]山西医科大学资产管理部,太原030001 [2]山西医科大学实验动物中心,实验动物与人类疾病动物模型山西省重点实验室,太原030001

出　　处：《中国比较医学杂志》2024年第3期1-9,共9页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(31970513);山西省中央引导地方科技发展资金项目(YDZJSX2022A060);山西省回国留学人员科研项目(2021-086);山西省科技创新人才团队项目(202204051002032)。

摘　　要：目的 本研究通过在细胞水平检测Gli2对口腔癌细胞Tca8113增殖、生长、迁移和侵袭的影响,明确Gli2调控口腔癌细胞迁移和侵袭的分子机制。方法 本研究利用siRNA抑制Tca8113细胞中Gli2的表达,通过CCK-8、平板克隆以及transwell小室实验分别检测Gli2对Tca8113细胞增殖、生长、迁移与侵袭的影响。进一步利用qRT-PCR与Western blot实验探究Gli2调控Tca8113细胞恶性增殖和转移机制。结果 口腔癌细胞Tca8113中Gli2的mRNA和蛋白表达升高。干扰Gli2表达可抑制Tca8113细胞增殖、生长、迁移及侵袭。进一步研究发现干扰Gli2表达可抑制Hedgehog(Hh)通路关键因子的mRNA与蛋白表达。此外,干扰Gli2表达可显著影响上皮间充质转化(epithelial mesenchymal transformation, EMT)通路关键因子的mRNA与蛋白表达。结论 口腔癌病变过程中Gli2被异常激活,干扰Gli2表达显著抑制口腔癌细胞的增殖、生长、迁移和侵袭。Gli2通过调控Hh通路与EMT通路,进而影响口腔癌细胞的迁移和侵袭。本研究为阐明口腔癌的发病机制提供新思路,并为口腔癌的临床治疗提供新视角。Objective To investigate the effects of Gli2 on the proliferation,growth,migration,and invasion of oral cancer cells(Tca8113)at the cellular level,and to clarify the molecular mechanism of how Gli2 regulation affects the migration and invasion of oral cancer cells.Methods Small interfering(si)RNA was used to inhibit Gli2 expression in Tca8113 cells.The effects of Gli2 on the proliferation,growth,migration,and invasion of Tca8113 cells were examined by CCK-8,plate cloning,and transwell chamber assay.Further qRT-PCR and Western blot assays were used to explore the mechanism of how Gli2 regulation effects the malignant proliferation and metastasis of Tca8113 cells.Results The mRNA and protein expression of Gli2 in oral cancer cells(Tca8113)increased.Interference of Gli2 expression inhibited the proliferation,growth,migration,and invasion of Tca8113 cells.Further experiments showed that interfering with Gli2 expression inhibited the mRNA and protein expression of key factors in the Hedgehog(Hh)pathway.In addition,interference of Gli2 expression significantly affected the mRNA and protein expression of key factors in epithelial mesenchymal transformation(EMT)pathways.Conclusions Gli2 is abnormally activated during oral cancer,and interference of Gli2 expression significantly inhibits the proliferation,growth,migration,and invasion of oral cancer cells.Gli2 influences the migration and invasion of oral cancer cells by regulating the Hh and EMT pathways.This study has provided a new way to elucidate the pathogenesis of oral cancer and new perspectives on the clinical treatment of oral cancer.

关 键 词：TCA8113细胞 口腔癌 GLI2 细胞迁移 细胞侵袭 HEDGEHOG通路 上皮间充质转化 

分 类 号：R-33[医药卫生]