精索静脉曲张合并勃起功能障碍动物模型的构建和评价  

Establishment and evaluation of an animal model of varicocele and erectile dysfunction

作　　者：陆杰[2] 李群生[1] 周磊 李东润 申振南 李凝华 陈冬 唐文平 朱璠宇 杨文涛 LU Jie;LI Qunsheng;ZHOU Lei;LI Dongrun;SHEN Zhennan;LI Ninghua;CHEN Dong;TANG Wenping;ZHU Fanyu;YANG Wentao(Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China;Guangxi University of Chinese Medicine,Nanning 530200;Nanxishan Hospital of Guangxi Zhuang Autonomous Region,Guilin 541000)

机构地区：[1]广西中医药大学附属瑞康医院,南宁530011 [2]广西中医药大学,南宁530200 [3]广西壮族自治区南溪山医院,广西桂林541000

出　　处：《中国比较医学杂志》2024年第3期18-26,共9页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(82160905,82260933);广西中医药大学研究生教育创新计划项目(YCBXJ2023019)。

摘　　要：目的通过手术诱导SD大鼠精索静脉曲张(VC),阿扑吗啡试验(APO试验)筛选VC后勃起功能障碍(ED)的大鼠,探讨建立VC合并ED模型的方法。方法将60只大鼠随机分为空白组、假手术组、模型组,每组20只;参照Turner法,部分结扎左侧肾静脉,诱导左侧VC;并进行3次APO试验,筛选诱导VC后出现ED的大鼠,观察并记录勃起次数、生殖器梳理次数、打哈欠次数;测量双侧精索静脉直径;对双侧睾丸和肾称重;HE染色观察阴茎和左睾的病理改变;统计模型组大鼠的成模率。结果模型组20只大鼠中有15只成功进行VC合并ED造模,成模率75%;造模后,模型组左侧精索静脉直径显著增加(P<0.01),且显著高于造模前(P<0.01),模型组右侧精索静脉直径增加(P<0.05),且高于造模前(P<0.05);模型组左侧睾丸与右侧睾丸相比,重量显著降低(P<0.01);各组双侧肾重量相比,组间及组内无明显差异(P<0.05);模型组勃起次数、打哈欠次数、生殖器梳理次数显著减少(P<0.01),且随造模时间递减;模型组左侧睾丸和阴茎病理改变显著。结论Turner法可以诱导大鼠精索静脉直径增加,引起睾丸损伤和重量下降,APO试验可以筛选诱导VC后出现ED的大鼠,二者相结合适用于构建与人类VC合并ED状态较相似的动物模型。Objective By employing surgically induced varicocele(VC)in SD rats and an apomorphine(APO)test,we screened rats with erectile dysfunction(ED)after VC and explored method to establish VC and ED models.Methods Sixty rats were randomly divided into Control,Sham,and Model groups with 20 rats in each group.Using the Turner method,we partially ligated the left renal vein to induce left VC three times.APO tests were conducted to screen rats with ED after inducing VC.The numbers of erections,genital grooming,and yawning were observed and recorded.The diameter of bilateral spermatic veins were measured.Both testises and kidneys were weighed.HE staining was used to observe pathological changes of penis and left testis.The success rate of modeling was calculated in the Model group.Results A VC and ED model was successfully established in 15 out of 20 rats in the Model group with a success rate of 75%.After modeling,the diameter of the left spermatic vein in the model group was increased significantly(P<0.01)and was significantly larger than that before modeling(P<0.01).The diameter of the right spermatic vein in the Model group was increased(P<0.05)and higher(P<0.05)than that before modeling.The weight of the left testis in the Model group was significantly decreased(P<0.01)compared with that of the right testis.No significant difference in the bilateral kidney weights were observed between or within groups(P<0.05).In the Model group,the numbers of erections,yawning,and genital grooming decreased significantly(P<0.01)with the time of modeling.Pathological changes of the left testis and penis were significant in the Model group.Conclusions The Turner method increases the diameter of the spermatic vein in rats,causing testis injury and weight loss,and APO tests can be used to screen rats with ED after VC induction.The combination of the two method is suitable to establish an animal model of VC with an ED status similar to humans.

关 键 词：精索静脉曲张 勃起功能障碍 造模 阿扑吗啡试验 

分 类 号：R-33[医药卫生]