大鼠急性梗阻性化脓性胆管炎模型的建立与评估  

Optimization and evaluation of acute obstructive suppurative cholangitis model in rats

作　　者：余奎[1] 梁晓强 韩冕 张静喆[3] YU Kui;LIANG Xiaoqiang;HAN Mian;ZHANG Jingzhe(Pudong Branch,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200126,China;Institute of Traditional Chinese Medicine and Surgery,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032)

机构地区：[1]上海中医药大学附属龙华医院浦东分院,上海200126 [2]上海中医药大学附属龙华医院中医外科研究所,上海200032 [3]上海中医药大学附属龙华医院,上海200032

出　　处：《中国比较医学杂志》2024年第3期45-50,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金青年项目(82004372);“龙医科技创新培育计划”临床医学引导项目(YD202206)。

摘　　要：目的建立一种稳定的大鼠急性梗阻性化脓性胆管炎模型,检测其病理生理指标,为急性胆管炎、胆汁淤积等疾病的研究提供稳定可靠的标准化动物模型。方法选用SPF级雄性SD大鼠,采用胆总管下段注射类毒素及胆总管结扎方式进行模型构建,评估大鼠造模前后的体重变化、死亡情况、肝功能主要指标变化及肝组织病理改变情况。结果造模后模型组大鼠体重明显降低;假手术组大鼠无死亡,肝功能无异常;模型组大鼠死亡3只,模型组死亡率12%,肝功能主要指标及肝病理呈现出明显的胆汁淤积及肝功能损伤性改变。结论本研究成功建立了大鼠急性梗阻性化脓性胆管炎模型,该模型具有操作简便、损伤小、死亡率低、造模成功率高等优势,可为多种常见疾病的机制研究和药物研发提供标准化的实验动物模型。Objective A stable model of acute obstructive suppurative cholangitis was established in rats to detect pathophysiological indexes and provide a reliable standardized animal model for the study of acute cholangitis and cholestasis.Methods SPF-grade male SD rats were selected,and the model was constructed via the injection of toxoid into the lower bile duct,followed by ligation of the common bile duct.Changes in body weight,mortality,major indexes of liver function,and histopathological changes in the liver were evaluated before and after modeling.Results After modeling,the body weight of rats in the model group decreased significantly.There were no deaths and no abnormalities of liver function in the sham-operation group.Three rats died in the model group,and the mortality rate of the model group was 12%.The main indexes of liver function and liver pathology showed obvious cholestasis and injurious changes to hepatic function in the model.Conclusions In this study,an acute obstructive suppurative cholangitis model rat was successfully established.The model has the advantages of ease of operation,minimal injury,low mortality,and a highly successful modeling rate,and it can provide a standardized experimental animal model for studying the mechanisms of and developing drugs for these common diseases.

关 键 词：急性梗阻性化脓性胆管炎 胆总管梗阻 动物模型 大鼠 

分 类 号：R-33[医药卫生]