散结祛瘢方对BALB/C裸鼠瘢痕疙瘩模型的作用及转化生长因子-β1的影响  

Effect of Sanjie Quban recipe on keloid model of BALB/C nude mice and influence on transforming growth factor-β1

作　　者：黄储涵 杨顶权[2] 钟施怡 吴瑞英 杨知山 方慧娟 刘青武 HUANGChuhan;YANGDingquan;ZHONGShiyi;WURuiying;YANGZhishan;FANGHuijuan;LIUQingwu(Beijing University of Chinese Medicine,Beijing 100029,China;China-Japan Friendship Hospital,Beijing 100029)

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院,北京100029

出　　处：《中国比较医学杂志》2024年第3期51-56,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金青年项目(82205115);中央高水平医院临床科研业务费资助(ZRJY2023-QM12);中日友好医院“菁英计划”人才培育工程资助(ZRJY2023-QM12)。

摘　　要：目的 探讨散结祛瘢方对瘢痕疙瘩裸鼠模型的作用及转化生长因子-β1(transforming growth factor-β1, TGF-β1)的影响。方法 将人手术切除后瘢痕疙瘩组织移植至6~8周龄健康SPF级BALB/C雌性裸鼠背部皮下,建立瘢痕疙瘩裸鼠模型,随机分成散结祛瘢方组、积雪苷片组和对照组,每组5只,分别用散结祛瘢方、积雪草苷片及无菌纯水进行干预,连续灌胃28 d后剥取瘢痕疙瘩组织,进行称重、苏木精伊红(hematoxylin eosin, HE)染色、Masson染色和免疫组化(TGF-β1)染色。比较3组间治疗前后的瘢痕疙瘩重量差值的差异,比较3组间治疗后的胶原纤维、成纤维细胞数量及TGF-β1表达的差异。结果 积雪草苷片组的瘢痕疙瘩重量差值大于对照组,且散结祛瘢方组与积雪草苷片组、对照组相比,散结祛瘢方组瘢痕疙瘩治疗前后重量差值最大(P<0.01);散结祛瘢方组较对照组胶原纤维疏松、数量减少,成纤维细胞数量减少;散结祛瘢方组较对照组TGF-β1表达减少(P<0.01)。结论 散结祛瘢方对瘢痕疙瘩具有一定的治疗作用,其机制可能是通过减少瘢痕疙瘩组织中TGF-β1的表达,从而减少成纤维细胞的增殖与细胞外基质的合成。该研究为临床用中药治疗瘢痕疙瘩提供了实验基础与理论依据。Objective This study aimed to investigate the therapeutic efficacy of Sanjie Quban recipe in a keloid nude mice model and its impact on transforming growth factor-β1(TGF-β1).Methods Keloid tissue after surgical resection was subcutaneously transplanted into the backs of healthy SPF BALB/C female nude mice,aged 6~8 weeks,and a keloid nude mice model was thus established.The mice were randomly divided into three groups,the Sanjie Quban recipe group,the Asiaticoside tablet group and the control gnup,with five in each group.They were respectively treated with Sanjie Quban recipe,Asiaticoside tablets,or sterile pure water.After 28 days of continuous gavage,the keloid tissue was exfoliated and weighed,and HE staining,Masson staining,and immunohistochemical staining for TGF-β1 were conducted.Differences in keloid weight between the three groups before and after treatment were compared,as were the differences in collagen fiber,fibroblast numbers,and TGF-β1 expression between the three groups after treatment.Results The difference in keloid weight before and after treatment in the Asiaticoside tablet group was greater than that of the control group,and the weight difference before and after treatment keloid treatment was the largest in the Sanjie Quban recipe group(P<0.01).Compared with the control group,collagen fibers in the Sanjie Quban recipe group were looser and less numerous,and fibroblasts were decreased in number.The expression of TGF-β1 in the Sanjie Quban recipe group was decreased compared with that of the control group(P<0.01).Conclusions Sanjie Quban recipe has certain therapeutic effects on keloids.The mechanism may involve reducing the expression of TGF-β1 in keloid tissue and thereby reducing the proliferation of fibroblasts and the synthesis of extracellular matrix.This study provides experimental and theoretical bases for the clinical treatment of keloids with Chinese medicine.

关 键 词：瘢痕疙瘩 散结祛瘢方 TGF-Β1 

分 类 号：R-33[医药卫生]