骆驼蓬在类风湿关节炎大鼠中的抗炎作用及对关节功能、关节滑膜组织NLRP3,ASC,Caspase-1的影响  

Anti-inflammatory Effect of Camel's Fur on Joint Function,Joint Synovial Tissue NLRP3,ASC,Caspase-1 in Rats with Rheumatoid Arthritis

作　　者：田新玮[1] 郑琦丽 王静 薛琴 艾比巴·阿不都拉 TIAN Xin-wei;ZHENG Qi-li;WANG Jing;XUE Qin;Abiba·Abdullah(Department of Rheumatology and Immunology,The Fifh Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,83001l,China)

机构地区：[1]新疆医科大学第五附属医院风湿免疫科,新疆乌鲁木齐830011

出　　处：《现代生物医学进展》2024年第4期624-629,共6页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区自然科学基金项目(2020D01C222)。

摘　　要：目的:探讨骆驼蓬在类风湿关节炎大鼠中的抗炎作用及对关节功能、关节滑膜组织NLRP3,ASC,Caspase-1的影响。方法:采用胶原诱导性关节炎模型。将大鼠分为对照组(假手术组)、模型组、骆驼蓬组、扶他林组、甲氨蝶呤组。关节围度及疼痛情况测定;关节滑膜组织HE染色;关节滑膜组织TUNEL染色观察凋亡;免疫组化检测大鼠关节滑膜组织NLRP3,ASC,Caspase-1表达。结果:与空白组相比,模型组、甲氨蝶呤组、骆驼蓬组、扶他林组左右踝关节肿胀程度和关节疼痛指数明显更高(P<0.05);与模型组相比,甲氨蝶呤组、骆驼蓬组、扶他林组左右踝关节肿胀程度和关节疼痛指数明显更低(P<0.05),且骆驼蓬组左右踝关节肿胀程度和关节疼痛指数明显低于甲氨蝶呤组、扶他林组;与空白组相比,模型组、甲氨蝶呤组、骆驼蓬组、扶他林组NLRP3、ASC、Caspase-1明显更高(P<0.05);与模型组相比,甲氨蝶呤组、骆驼蓬组、扶他林组NLRP3、ASC、Caspase-1明显更低(P<0.05),且骆驼蓬组NLRP3、ASC、Caspase-1明显低于甲氨蝶呤组、扶他林组;与空白组相比,模型组、甲氨蝶呤组、骆驼蓬组、扶他林组凋亡率明显更高(P<0.05);与模型组相比,甲氨蝶呤组、骆驼蓬组、扶他林组凋亡率明显更低(P<0.05),且骆驼蓬组凋亡率明显低于甲氨蝶呤组、扶他林组;与模型组比较,甲氨蝶呤组、扶他林组、骆驼蓬组三组关节软骨面均较光滑,滑膜组织增生及炎细胞浸润程度均减轻(P<0.05)。结论:骆驼蓬可通过调节滑膜组织NLRP3炎症小体的表达抑制滑膜组织炎症反应,进而达到改善关节肿胀和疼痛的目的。Objective:To investigate the anti-inflammatory effects of camel's bark on joint function,joint synovial tissue NLRP3,ASC,Caspase-1 in rats with rheumatoid arthritis.Methods:Collagen induced arthritis model was adopted.The rats were divided into control group(sham operation group),model group,camelback group,Voltaline group and methotrexate group.Measurement of joint circumference and pain;Joint synovial tissue HE staining;The apoptosis of synovial tissue was observed by TUNEL staining.The expressions of NLRP3,ASC,Caspase-1 in synovial tissues of rats were detected by immunohistochemistry.Results:Compared with blank group,the swelling degree and joint pain index of left and right foot joint in model group,methotrexate group,camelback group and voltaline group were higher(P<0.05).Compared with model group,the left and right foot joint swelling degree and joint pain index in methotrexate group,camphorus group and voltaalin group were lower(P<0.05),and the left and right foot joint swelling degree and joint pain index in camphorus group were significantly lower than those in methotrexate group and Voltaalin group.Compared with blank group,NLRP3,ASC and Caspase-1 in model group,methotrexate group,camelback group and Voltaalin group were higher(P<0.05).Compared with model group,NLRP3,ASC and Caspase-1 were lower in Methotrexate group,Camphorus group and Voltaalin group(P<0.05),and NLRP3,ASC and Caspase-1 in camphorus group were significantly lower than those in methotrexate group and Voltaalin group.Compared with blank group,the apoptosis rate of model group,methotrexate group,camelaria group and voltaline group was higher(P<0.05).Compared with model group,the apoptosis rate of methotrexate group,camel peng group and voltaalin group was significantly lower(P<0.05),and the apoptosis rate of camel peng group was significantly lower than that of Methotrexate group and Voltaalin group.Compared with the model group,the articular cartilage surface of Methotrexate group,Voltaline group and camphor group were smooth,and the de

关 键 词：骆驼蓬 类风湿关节炎 关节功能 NLRP3 ASC CASPASE-1 

分 类 号：R-33[医药卫生] R684.3