桃红四物汤对缺糖缺氧大鼠脑微血管内皮细胞VEGF、VEGFR-2、Akt基因及蛋白表达的作用机制研究  

Study on the Mechanism of Taohong Siwu Decoction on the Expression of VEGF,VEGFR-2,Akt Gene and Protein in Brain Microvascular Endothelial Cells of Rats with Oxygen Glucose Deprivation

作　　者：郭少英[1] 闫建军[1] 李媛[1] 娄重章[1] 乔磊 李昆城[1] 王静[2] GUO Shao-ying;YAN Jian-jun;LI Yuan;LOU Chong-zhang;QIAO Lei;LI Kun-cheng;WANG Jing(Department of Traditional Chinese Medicine,Handan Central Hospital of Hebei Province,Handan,Hebei,056001,China;Department of Neurology,Handan Central Hospital of Hebei Province,Handan,Hebei,056001,China)

机构地区：[1]河北省邯郸市中心医院中医科,河北邯郸056001 [2]河北省邯郸市中心医院神经内科,河北邯郸056001

出　　处：《现代生物医学进展》2024年第4期636-640,共5页Progress in Modern Biomedicine

基　　金：河北省2022年度医学科学研究课题计划项目(20220563)。

摘　　要：目的:探讨桃红四物汤对缺糖缺氧(OGD)大鼠脑微血管内皮细胞(r BMECs)血管内皮生长因子(VEGF)、血管内皮生长因子受体2(VEGFR-2)、蛋白激酶B(Akt)基因及蛋白表达的影响及其作用机制。方法:取r BMECs进行培养并随机分为正常组培养、模型组培养、桃红四物汤(0.4 mg/mL)组和桃红四物汤(0.8 mg/mL)组,除正常组外,其余三组采用氧糖剥夺实验构建OGD损伤模型。桃红四物汤组在造模前1 h按0.4 mg/mL、0.8 mg/mL浓度加入桃红四物汤,而正常组及模型组则加入等量体积生理盐水。实时定量聚合酶链反应(qRT-PCR)、蛋白免疫印迹试验检测VEGF、VEGFR-2、Akt基因及蛋白表达情况。结果:与正常组比较,模型组细胞凋亡率显著升高(P<0.05)。与模型组比较,桃红四物汤(0.4 mg/mL)组和桃红四物汤(0.8 mg/mL)组均降低,且以桃红四物汤(0.8 mg/mL)组最为显著(P<0.05)。与正常组比较,模型组r BMECs中Akt、VEGFR-2、VEGF相对m RNA和蛋白表达水平显著升高(P<0.05)。与模型组比较,桃红四物汤(0.4 mg/mL)组和桃红四物汤(0.8 mg/mL)组显著降低,且以桃红四物汤(0.8 mg/mL)组最为显著(P<0.05)。结论:桃红四物汤可能通过降低OGD条件下r BMECs中VEGF、VEGFR-2、Akt基因及蛋白表达水平,从而发挥抑制内皮细胞的凋亡,保持内皮细胞的完整性,这可能是桃红四物汤对脑保护的机制之一。Objective:To investigate the effect of Taohong Siwu decoction on the expression of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR-2),protein kinase B(Akt)gene and protein in brain microvascular endothelial cells(rBMECs)of rats with oxygen glucose deprivation(OGD)and its mechanism.Methods:The rBMECs were cultured and randomly divided into normal group,model group,Taohong Siwu decoction(0.4 mg/mL)group and Taohong Siwu decoction(0.8 mg/mL)group.In addition to the normal group,the other three groups used oxygen glucose deprivation experiment to construct OGD injury model.Taohong Siwu decoction group was added with Taohong Siwu decoction at a concentration of 0.4 mg/mL and 0.8 mg/mL 1 h before modeling,while the normal group and the model group were added with the same volume of normal saline.Real-time quantitative polymerase chain reaction(qRT-PCR)and Western blotting were used to detect the expression of VEGF,VEGFR-2 and Akt genes and proteins.Results:Compared with the normal group,the apoptosis rate of the model group was significantly increased(P<0.05).Compared with the model group,the Taohong Siwu decoction(0.4 mg/mL)group and Taohong Siwu decoction(0.8 mg/mL)group decreased,and the Taohong Siwu decoction(0.8 mg/mL)group was the most significant(P<0.05).Compared with the normal group,the relative mRNA and protein expression levels of Akt,VEGFR-2 and VEGF in rBMECs of the model group were significantly increased(P<0.05).Compared with the model group,the Taohong Siwu decoction(0.4 mg/mL)group and Taohong Siwu decoction(0.8 mg/mL)group were significantly decreased,and the Taohong Siwu decoction(0.8 mg/mL)group was the most significant.Conclusion:Taohong Siwu decoction may inhibit the apoptosis of endothelial cells and maintain the integrity of endothelial cells by reducing the expression levels of VEGF,VEGFR-2 and Akt genes and proteins in rBMECs under OGD conditions,which may be one of the mechanisms of Taohong Siwu Decoction on brain protection.

关 键 词：桃红四物汤 缺糖缺氧 大鼠 脑微血管内皮细胞 VEGF VEGFR-2 Akt 

分 类 号：R-33[医药卫生] R743R243