血清zonulin、MCP-1、HIF-1α与克罗恩病患者疾病活动度和肠道菌群的相关性分析及其鉴别价值分析  

Correlation Analysis of Serum zonulin,MCP-1 and HIF-1αwith Disease Activity and Intestinal Microbiota in Patients with Crohn's Disease and Their Differential Value Analysis

作　　者：罗芙蓉 林奕[1] 王叶青 何盛伊 段文丽[1] LUO Fu-rong;LIN Yi;WANG Ye-qing;HE Sheng-yi;DUAN Wen-li(Department of Anorectal,First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha,Hunan,410000,China)

机构地区：[1]湖南中医药大学第一附属医院肛肠科,湖南长沙410000

出　　处：《现代生物医学进展》2024年第4期680-684,655,共6页Progress in Modern Biomedicine

基　　金：湖南省中医药管理局科研计划项目重点课题(201604)。

摘　　要：目的:探讨血清连蛋白(zonulin)、单核细胞趋化蛋白-l(MCP-1)、缺氧诱导因子-1α(HIF-1α)与克罗恩病(CD)患者疾病活动度和肠道菌群的相关性,分析zonulin、MCP-1、HIF-1α鉴别重度CD的价值。方法:选择2019年1月至2022年1月湖南中医药大学第一附属医院收治的162例CD患者,根据克罗恩病活动指数(CDAI)将CD患者分为重度组、中度组和轻度组,分别为42例、69例和51例,另选同期151例健康体检志愿者为对照组。检测CD患者和对照组血清zonulin、MCP-1、HIF-1α水平,Pearson相关分析zonulin、MCP-1、HIF-1α与CDAI评分、肠道菌群相对丰度的相关性。受试者工作特征曲线(ROC)分析zonulin、MCP-1、HIF-1α鉴别重度CD的价值。结果:重度组、中度组、轻度组血清zonulin、MCP-1、HIF-1α水平均高于对照组(P<0.05)。重度组血清zonulin、MCP-1、HIF-1α水平、大肠杆菌、沃氏嗜胆菌、Atopobium parvulum相对丰度高于中度组和轻度组,且中度组高于轻度组(P<0.05);普拉梭菌相对丰度轻度组高于中度组,中度组高于重度组(P<0.05)。CD患者血清zonulin、MCP-1、HIF-1α水平与CDAI评分、大肠杆菌、沃氏嗜胆菌、Atopobium parvulum相对丰度呈正相关,与普拉梭菌相对丰度呈负相关(P<0.05)。联合zonulin、MCP-1、HIF-1α鉴别重度CD的曲线下面积为0.856,高于单独zonulin、MCP-1、HIF-1α的0.706、0.693、0.747。结论:CD患者血清zonulin、MCP-1、HIF-1α水平均升高,且与CD疾病活动度增加和肠道菌群紊乱有关,可能是重度CD诊断的潜在生物学标志物。Objective:To investigate the correlation of serum zonulin,monocyte chemotactic protein-1(MCP-1),hypoxia-inducible factor-1α(HIF-1α)with disease activity and intestinal microflora in patients with Crohn's disease(CD),and to analyze the value of zonulin,MCP-1 and HIF-1αin the identification of severe CD.Methods:162 patients with CD who were admitted to The First Affiliated Hospital of Hunan University of Chinese Medicinel from January 2019 to January 2022 were selected.According to Crohn's disease activity index(CDAI),patients with CD were divided into severe group,moderate group and mild group,with 42,69,and 51 cases,respectively,another 151 physical examination volunteers during the same period were selected as the control group.The levels of serum zonulin,MCP-1 and HIF-1αin the patients with CD and control group were detected.Pearson correlation analysis was performed to analyze the correlation between zonulin,MCP-1 and HIF-1αwith CDAI score and the relative abundance of intestinal flora.Receiver operating characteristic curve(ROC)was used to analyze the value of zonulin,MCP-1 and HIF-1αin identifying severe CD.Results:The levels of serum zonulin,MCP-1 and HIF-1αin the severe group,moderate group and mild group were higher than those in the control group(P<0.05).The levels of serum zonulin,MCP-1,HIF-1α,and the relative abundance of Escherichia coli,Choleosinophilus wardeni,and Atopobium parvulum in the severe group were higher than those in the moderate group and mild group,and the moderate group was higher than the mild group(P<0.05),and the relative abundance of Clostridium pratense in the mild group was higher than that in the moderate group,and moderate group was higher than the severe group(P<0.05).The levels of serum zonulin,MCP-1 and HIF-1αin patients with CD were positively correlated with CDAI score,the relative abundance of Escherichia coli,Choleosinophilus wardeni and Atopobium parvulum,and negatively correlated with the relative abundance of Clostridium pratense(P<0.05).The area under curve(

关 键 词：克罗恩病 ZONULIN MCP-1 HIF-1Α 疾病活动度 肠道菌群 相关性 鉴别价值 

分 类 号：R574[医药卫生—消化系统]