角膜缘微环境细胞的分离培养研究进展  

Advances in the isolation and culture of limbal niche cells

作　　者：肖宇婷(综述) 谢华桃 张明昌(审校)[1] Xiao Yuting;Xie Huatao;Zhang Mingchang(Department of Ophthalmology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022

出　　处：《中华实验眼科杂志》2024年第4期387-392,共6页Chinese Journal Of Experimental Ophthalmology

基　　金：国际科技合作重点项目计划(2017YFE0103500);中央高校基本科研基金(HUST:2019kfyXMBZ065)。

摘　　要：角膜缘微环境细胞(LNCs)是位于角膜缘、与角膜缘上皮干细胞(LESCs)紧密相连的一类间充质干细胞,能同时表达间充质标志物和多种胚胎干细胞标志物,对调节LESCs的静息、自我更新以及分化状态起着重要作用。近年研究表明,LNCs可通过胶原酶消化法、上皮块消化法、中性蛋白酶-胶原酶消化法以及组织块培养法进行体外分离培养,通过3D Matrigel共培养、Transwell共培养可研究LNCs与LESCs之间的相互作用关系。LNCs与LESCs可通过SDF-1/CXCR4、Notch、BMP、Wnt、Sonic Hedgehog、KIT/AKT等多种信号通路以及神经生长因子、角质形成细胞因子、胰岛素样生长因子等多种细胞因子相互作用。LNCs现已成为角膜上皮组织工程、眼表重建以及角膜再生等研究中的一大热点。本文就LNCs的研究背景、体外分离培养方法、与LESCs的相互作用机制及其应用前景等进行综述。Limbal niche cells(LNCs)in the limbal niche,a type of mesenchymal stem cells closely associated with limbal epithelial stem/progenitor cells(LESCs),heterogeneously express both mesenchymal and putative embryonic stem cell markers and play a critical role in regulating the quiescence,self-renewal,and differentiation of LESCs.Previous studies have shown that LNCs can be isolated by collagenase,dispase,dispase-collagenase and explant culture.Transwell and 3D Matrigel coculture are widely used in ex vivo studies of LNCs and LESCs,and the interactive mechanism may include SDF-1/CXCR4,Notch,BMP,Wnt,Sonic Hedgehog,and KIT/AKT signaling pathways,and various cytokines such as nerve growth factor,keratinocyte growth factor,and insulin-like growth factor.LNCs have become a hot topic in corneal epithelial tissue engineering,ocular surface reconstruction,and corneal regeneration.This review provides an overview of the research background,isolation and culture methods,interaction mechanism of LNCs with LESCs,and its application prospects.

关 键 词：角膜 角膜缘微环境细胞 角膜缘上皮干细胞 角膜缘干细胞缺乏症 组织工程 眼表重建 角膜再生 

分 类 号：R77[医药卫生—眼科]