基于HPLC指纹图谱和多组分含量测定结合化学模式识别研究的龙眼核质量评价  

Quality evaluation of seeds of Dimocarpus longan Lour.based on HPLC fingerprint and multi-component content determination combined with chemical pattern recognition

作　　者：王艺洁 蒋佳丽 陈彦洁 罗鑫 蒋学青 王志萍 谢谭芳 WANG Yi-jie;JIANG Jia-li;CHEN Yan-jie;LUO Xin;JIANG Xue-qing;WANG Zhi-ping;XIE Tan-fang(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200;Collaborative Innovation Center of Research on Functional Ingredients from Agricultural Residues(Guangxi University of Chinese medicine),Nanning 530200;Key Laboratory of Generic Technology Research and Development of Traditional Chinese Medicine Preparations in Colleges and Universities in Guangxi,Nanning 530200)

机构地区：[1]广西中医药大学药学院,南宁530200 [2]农作物废弃物功能成分研究协同创新中心,南宁530200 [3]广西高校中药制剂共性技术研发重点实验室,南宁530200

出　　处：《中南药学》2024年第4期1059-1064,共6页Central South Pharmacy

基　　金：农作物废弃物功能成分研究协同创新中心资助课题(No.CICAR 2023-P2)。

摘　　要：目的 建立龙眼Dimocarpus longan Lour.核HPLC指纹图谱及含量测定方法。方法 建立13批龙眼核指纹图谱,结合指纹图谱相似度评价及化学模式识别研究对龙眼核质量进行分析,并测定其中没食子酸、柯里拉京、鞣花酸的含量。结果 13批龙眼核样品相似度在0.919~0.999,标定共有峰11个,并指认出3种成分;聚类分析(HCA)将13批龙眼核聚为3类,主成分分析(PCA)提取到3个主成分,正交偏最小二乘判别分析(OPLS-DA)筛选出5个差异性成分;定量分析得到13批龙眼核中没食子酸、柯里拉京金、鞣花酸的含量范围依次为0.65~1.8 mg·g^(-1)、3.02~5.46 mg·g^(-1)、3.18~4.49 mg·g^(-1)。结论 建立的HPLC指纹图谱及含量测定方法重复性、稳定性良好,能够为龙眼核质量评价提供参考。Objective To establish the HPLC fingerprint of seeds of Dimocarpus longan Lour.and to determine differential components.Methods The quality of seeds of Dimocarpus longan was analyzed by establishing the fingerprints of 13 batches of samples combined with similarity evaluation of the fingerprints and chemical pattern recognition.The content of gallic acid,corilagin,and ellagic acid was determined.Results The similarity of 13 batches of seeds of Dimocarpus longan ranged from 0.919 to 0.999,with a total of 11 peaks confirmed and 3 components identified.HCA clustered 13 batches of seeds of Dimocarpus longan into 3 categories,PCA extracted 3 principal components,and OPLS-DA screened 5 differential components.Quantitative analysis showed that the content ranges of gallic acid,corilagin,and ellagic acid were 0.65~1.8 mg·g^(-1),3.02~5.46 mg·g^(-1),and 3.18~4.49 mg·g^(-1),respectively.Conclusion The HPLC fingerprint and content determination method are stable and repeatable,which can provide reference for the quality evaluation of seeds of Dimocarpus longan.

关 键 词：龙眼核 指纹图谱 化学模式识别研究 含量测定 

分 类 号：R284[医药卫生—中药学] O657.7[医药卫生—中医学]