Chemoselective labeling-based spermatozoa glycan imaging reveals abnormal glycosylation in oligoasthenotspermia  

作　　者：Lijia Xu Tong Zhong Wei Zhao Bing Yao Lin Ding Huangxian Ju 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China [2]Department of Reproductive Medical Center,Jinling Hospital,School of Medicine,Nanjing University,Nanjing 210002,China [3]Chemistry and Biomedicine Innovation Center(ChemBIC),Nanjing University,Nanjing 210023,China [4]Center of Clinical Laboratory Science,The Affiliated Cancer Hospital of Nanjing Medical University&Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research,Nanjing 210009,China

出　　处：《Chinese Chemical Letters》2024年第4期475-480,共6页中国化学快报（英文版）

基　　金：the support from the National Natural Science Foundation of China (Nos.21974067, 22274073, 81971373 and 82001535);the National Key Research and Development Program of China (No.2018YFC1004700);Fundamental Research Funds for the Central Universities (Nos.020514380309,021414380502 and 2022300324);the State Key Laboratory of Analytical Chemistry for Life Science (Nos.5431ZZXM2305 and 5431ZZXM2204)。

摘　　要：Spermatogenesis, maturation, capacitation and fertilization are precisely regulated by glycosylation. However, the relationship between altered glycosylation patterns and the onset and development of reproductive disorders is unclear, mainly limited by the lack of in situ imaging techniques for spermatozoa glycosylation. We developed an efficient and highly specific spermatozoa glycan imaging technique based on the robust chemoselective labeling of sialic acid(Sia) and N-acetyl-D-galactosamine(Gal/GalNAc). We further proposed a “tandem glycan chemoselective labeling” strategy to achieve simultaneous imaging of two types of glycans on spermatozoa. We applied the developed method to the spermatozoa from oligozoospermic patients and diabetic mice and found that these spermatozoa showed higher levels of Sia and Gal/Gal NAc expression than the normal groups. Moreover, spermatozoa from diabetic mice showed a severe decrease in number, viability, and forward motility, suggesting that in vivo glucose metabolism disorders may lead to an elevated level of spermatozoa glycosylation and have a correlation with the development of oligoasthenotspermia. Our work provides a research tool to reveal the relationship between glycosylation modification and spermatozoa quality, and a promising clue for the development of glycan-based reproductive markers.

关 键 词：SPERMATOZOA GLYCAN Chemoselective labeling Oligoasthenotspermia Fluorescence imaging 

分 类 号：O657.3[理学—分析化学] R698.2[理学—化学]