荧光假单胞菌Pseudomonas fluorescens RN-88抑菌特性研究  

Antifungal Characters of Pseudomonas fluorescens RN-88

作　　者：朱李霞 周蓉蓉 张赛男 毛洪刚 夏禧龙 李春雨 尹冬敏 任建军 ZHU Lixia;ZHOU Rongrong;ZHANG Sainan;MAO Honggang;XIA Xilong;LI Chunyu;YIN Dongmin;REN Jianjun(Changzhou Key Laboratory of Biomass Green,Safe and High Value Utilization Technology/National-Local Joint EngineeringResearch Center of Biomass Refining and High-Quality Utilization/Institute of Urban and Rural Mining,Changzhou University,Changzhou,Jiangsu 213164;School of Pharmacy/School of Biological and Food Engineering,Changzhou University,Changzhou,Jiangsu 213164;Beijing General Station of Animal Husbandry,Beijing 100107)

机构地区：[1]常州大学城乡矿山研究院,生物质高效炼制及高质化利用国家地方联合工程中心,常州市生物质绿色安全高值利用技术重点实验室,江苏常州213164 [2]常州大学药学院,生物与食品工程学院,江苏常州213164 [3]北京市畜牧总站,北京100107

出　　处：《中国农学通报》2024年第11期22-27,共6页Chinese Agricultural Science Bulletin

基　　金：贵州省科技计划项目“养殖废弃物无害化处理与资源化利用技术集成应用研究”(黔科合支撑【2022】一般175);“江苏省高等学校基础科学(自然科学)研究重大项目”(23KJA610001);北京市家禽创新团队(BAIC062023)。

摘　　要：本研究旨在利用拮抗菌防治植物病害,为桃的病害生物防治提供更多可行性的方案。以前期筛选获得的一株桃褐腐病拮抗细菌荧光假单胞菌(Pseudomonas fluorescens)RN-88为试验材料,采用杯碟法测定发酵液对桃褐腐菌(Monilinia fructicol)的抑菌活性,并结合抑菌圈法,研究了该菌在pH、温度、紫外线(UV)等不同环境中的稳定性。实验结果表明,细菌RN-88能显著降低桃褐腐病的发病率,其发酵液对褐腐病菌(M.fructicol)的抑制率可达44.69%。在过滤除菌后,相对于高温灭菌,发酵液中抑菌物质的损失较小。在pH 7.5~9.0的碱性溶液中,发酵液的抑菌活性有较好的稳定性,但在酸性溶液(3.0≤pH≤6.5)中处理后,其抑菌能力减弱。发酵液的抑菌率几乎不受温度和紫外线(UV)的影响。Pseudomonas fluorescens RN-88细菌具有较好的环境稳定性,具有开发成桃褐腐病生物拮抗剂的潜在价值,本研究为荧光假单胞菌作为新型生防菌的应用提供了理论依据,同时为实现桃病害的生物防治提供了更多可行性的方案。This study aims to use antagonistic bacteria to control plant diseases,and provide more feasible biological control methods for the diseases control of peach.A strain Pseudomonas fluorescens RN-88 obtained previously in the authors lab was used to determine the inhibitory activity of the fermentation broth against Monilinia fructicol,which was determined by the cup and saucer method.The stability of the broth under different environments such as pH,temperature,and ultraviolet(UV)light was evaluated by the inhibition circle method.The results showed that the antagonistic bacterium RN-88 reduced the incidence of brown rot in peaches,and its fermentation broth inhibited M.fructicol up to 44.69%,and the loss of fungal inhibitory ability of the fermentation broth was effectively reduced by filtration compared to autoclaving.The inhibition activity of the fermentation broth was stable in alkaline solutions at pH 7.5-9.0,and was reduced in acidic solutions(3.0≤pH≤6.5).There was no effect on the inhibition rate of the fermentation broth by temperature and ultraviolet(UV)light.All above,fermentation broth of Pseudomonas fluorescens RN-88 has good environmental stability which has the potential to be developed to a novel fungicide.This study also provides a theoretical basis for the application of Pseudomonas fluorescens.

关 键 词：桃褐腐病 生防细菌资源 抑菌活性 环境稳定性 荧光假单胞菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]