耐糖β-葡萄糖苷酶基因的挖掘、突变及应用研究进展  

Research Progress in Mining,Mutation and Application of Glucose-tolerantβ-glucosidase Genes

作　　者：张婷 宁远妮 冯家勋[2] 赵帅[2] ZHANG Ting;NING Yuanni;FENG Jiaxun;ZHAO Shuai(College of Food and Quality Engineering,Nanning University,Nanning,530200;College of Life Science and Technology,Guangxi University,Nanning,530004)

机构地区：[1]南宁学院食品与质量工程学院,南宁530200 [2]广西大学生命科学与技术学院,南宁530004

出　　处：《基因组学与应用生物学》2024年第3期377-386,共10页Genomics and Applied Biology

基　　金：2023年度广西高校中青年教师科研基础能力提升项目(2023KY1871);2023年南宁市“邕江计划”青年人才专项(RC20230202)共同资助。

摘　　要：降低木质纤维素生物炼制的用酶成本,对推动木质纤维素的绿色利用至关重要。作为纤维素水解过程中的限速酶,β-葡萄糖苷酶酶解活性受高浓度葡萄糖的胁迫抑制。目前,挖掘和突变耐糖β-葡萄糖苷酶的基因是解决这一问题的重要策略。本综述总结了近年来在耐糖β-葡萄糖苷酶基因的挖掘、酶耐糖分子机制的分析、旨在提高酶耐糖性能的酶基因突变以及基于β-葡萄糖苷酶基因的微生物菌株基因工程改造等方面所取得的进展,并进行了展望。本综述为构建高产耐糖β-葡萄糖苷酶的基因工程菌株提供参考,这对推动木质纤维素生物炼制工业化进程具有重要意义。Reducing enzyme cost used in lignocellulosic biorefineries is important to promote the green utilization of lignocellulose.β-glucosidase,a rate-limiting enzyme during enzymatic hydrolysis of cellulose,is inhibited by a high concentration of glucose.Mining and mutating the genes of glucose-tolerantβ-glucosidases are important strategies.This paper summarizes the progress made in recent years in mining glucose-tolerantβ-glucosidase genes,analyzing the molecular mechanism of enzyme tolerance to glucose,mutation ofβ-glucosidase gene to improve the glucose-tolerance,and genetic engineering of modified microbial strains based onβ-glucosidase genes,and then perspectives.This paper will provide references for constructing genetically engineered strains with a high yield of glucose-tolerantβ-glucosidase,which is of great significance to the development of lignocellulosic biorefineries.

关 键 词：生物炼制 Β-葡萄糖苷酶 葡萄糖胁迫 工程菌 

分 类 号：Q55[生物学—生物化学] Q78