佛手和山金柑果形相关基因CRC的TRV-VIGS体系的构建研究  

Research on the Establishment of TRV-VIGS System of Fruit Shape-Related Genes CRC in Fingered Citron and Hongkong Kumquat

作　　者：尉俊海 吴中原 王小青 梅煜琳 陈文荣[1] 廖芳蕾[1,2] 郭卫东 WEI Junhai;WU Zhongyuan;WANG Xiaoqing;MEI Yulin;CHEN Wenrong;LIAO Fanglei;GUO Weidong(College of Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Jinhua,Zhejiang 321004,China)

机构地区：[1]浙江师范大学生命科学学院,浙江金华321004 [2]浙江省特色经济植物生物技术研究重点实验室,浙江金华321004

出　　处：《园艺学报》2024年第4期715-726,共12页Acta Horticulturae Sinica

基　　金：金华市科学技术研究计划项目(2020-2-006)。

摘　　要：以芸香科的佛手(Citrus medica L.var.sarcodactylis Swingle)和山金柑(Fortunella hindsii Swingle)花期叶片为材料,以果实发育相关的CRC基因为对象,利用烟草脆裂病毒(tobacco rattle virus,TRV)研究构建病毒诱导的基因沉默(virus-induced gene silencing,VIGS)技术体系,为基因功能研究提供有效简便的方法。待沉默的佛手植株剪枝、抹除花芽后,将CRC基因的沉默载体两次注射于佛手和山金柑的顶部叶片,注射间隔15~30 d。在注射后25~115 d内,佛手新生叶、新生花、果中的CmsCRC表达量均显著下调,且沉默植株的果形聚拢,果实指状结构数增加。CRC基因在佛手中的沉默有效时间是侵染后25~115 d,而在山金柑中沉默有效时间是25~105 d。Using fingered citron(Citrus medica L.var.sarcodactylis Swingle)and Hongkong kumquat(Fortunella hindsii Swingle)leaves from the Rutaceae family as materials,the role of CRC genes in fruit development were investigated.To achieve this,the tobacco rattle virus(TRV)to create a virus-induced gene silencing(VIGS)technology system were employed,which provided a simple and effective method for studying gene function.The silencing vector targeting the CRC gene was injected twice into the top leaves of fingered citron and Hongkong kumquat,with a time interval of 15–30 days.Between 25 and 115 days after the injections,a significant down-regulation of CmsCRC expression was observed in the neonatal leaves,neonatal flowers,and fruits of Fingered citron.The silenced plants exhibited clustered fruit shapes and an increased number of fruit fingers.The silencing effect of the CRC gene in fingered citron lasted for 25–115 days,while in Hongkong kumquat,it lasted for 25–105 days.

关 键 词：VIGS 佛手 山金柑 CRC 果形 

分 类 号：S666.9[农业科学—果树学]