Salidroside Ameliorates Lung Injury Induced by PM_(2.5) by Regulating SIRT1-PGC-1αin Mice  

作　　者：LI Xiao Hong LIU Yu Mei SHAN Hui TAN Jin Feng ZHOU Jian SONG Yuan Jin LI Si Qi LIU Chen XU Dong Qun YU Li LI Wan Wei 

机构地区：[1]School of Public Health,Shandong Second Medical University,Weifang 261053,Shandong,China [2]Key Laboratory of Health Inspection and Quarantine,Weifang 261053,Shandong,China [3]Weifang Environmental Monitoring Station,Weifang 261042,Shandong,China [4]Department of Orthopedics,The 80th Army Hospital of PLA,Weifang 261000,Shandong,China [5]Environment and Health Related Product Safety Institute of Chinese Center for Disease Control and Prevention,Beijing 100021,China [6]School of Basic Medicine,Shandong Second Medical University,Weifang 261053,Shandong,China

出　　处：《Biomedical and Environmental Sciences》2024年第4期367-376,共10页生物医学与环境科学（英文版）

基　　金：supported by Shandong Provincial Natural Science Foundation,China(No.ZR2020MH336);Weifang Science and Technology Development Plan Project(NO.2022GX015,NO.2022GX010).

摘　　要：Objective This study aimed to clarify the intervention effect of salidroside(SAL)on lung injury caused by PM_(2.5) in mice and illuminate the function of SIRT1-PGC-1ɑaxis.Methods Specific pathogen-free(SPF)grade male C57BL/6 mice were randomly assigned to the following groups:control group,SAL group,PM_(2.5) group,SAL+PM_(2.5) group.On the first day,SAL was given by gavage,and on the second day,PM_(2.5) suspension was given by intratracheal instillation.The whole experiment consist of a total of 10 cycles,lasting 20 days.At the end of treatment,blood samples and lung tissues were collected and analyzed.Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy.The expression of inflammatory,antioxidants,apoptosis,and SIRT1-PGC-1ɑproteins were detected by Western blotting.Results Exposure to PM_(2.5) leads to obvious morphological and pathologica changes in the lung of mice.PM_(2.5) caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1,Nrf2,SOD2,SIRT1 and PGC-1ɑ,and an increase in the protein expressions of IL-6,IL-1β,Bax,caspase-9 and cleaved caspase-3.However,SAL reversed the aforementioned changes caused by PM_(2.5) by activating the SIRT1-PGC-1α pathway.Conclusion SAL can activate SIRT1-PGC-1ɑ to ameliorate PM2.5-induced lung injury.

关 键 词：PM_(2.5) SALIDROSIDE Oxidative stress Inflammation Apoptosis SIRT1-PGC-1α 

分 类 号：R285.5[医药卫生—中药学]