Barley Protein LFBEP-C1 from Lactiplantibacillus plantarum dy-1 Fermented Barley Extracts by Inhibiting Lipid Accumulation in a Caenorhabditis elegans Model  

作　　者：ZHANG Jia Yan LIU Meng Ting LIU Yu Hao DENG Huan BAI Juan XIE Jian Hua XIAO Xiang 

机构地区：[1]School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu,China [2]State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,Jiangxi,China

出　　处：《Biomedical and Environmental Sciences》2024年第4期377-386,共10页生物医学与环境科学（英文版）

基　　金：supported by the priority academic program development of Jiangsu Higher education institutions;the National Natural Science Foundation of China [31801538, 32072200];China Postdoctoral Science Foundation[2019M651747].

摘　　要：Objective This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans(C.elegans).Methods In this study,the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on C.elegans.The worms were fed Escherichia coli OP50(E.coli OP50),glucose,and different concentrations of LFBEP-C1.Body size,lifespan,movement,triglyceride content,and gene expression were analyzed.The results were analyzed using ANOVA and Tukey's multiple comparison test.Results Compared with the model group,the head-swing frequency of C.elegans in the group of LFBEP-C1 at 20μg/mL increased by 33.88%,and the body-bending frequency increased by 27.09%.This indicated that LFBEP-C1 improved the locomotive ability of C.elegans.The average lifespan of C.elegans reached 13.55 days,and the body length and width of the C.elegans decreased after LFBEP-C1 intake.Additionally,LFBEP-C1 reduced the content of lipid accumulation and triglyceride levels.The expression levels of sbp-1,daf-2,and mdt-15 significantly decreased,while those of daf-16,tph-1,mod-1,and ser-4 significantly increased after LFBEP-C1 intake.Changes in these genes explain the signaling pathways that regulate lipid metabolism.Conclusion LFBEP-C1 significantly reduced lipid deposition in C.elegans fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development,lifespan,and exercise behavior of C.elegans.In addition,LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein,insulin,and 5-hydroxytryptamine signaling pathways.

关 键 词：LFBEP-C1 Fermentation Protein Caenorhabditis elegans Lipid accumulation Signaling pathway 

分 类 号：TS201.3[轻工技术与工程—食品科学]