玉米须多糖的理化性质和体外生物活性研究  

Study on the physicochemical characteristics and in vitro bioactivities of corn silk polysaccharides

作　　者：况慧云 孙萍东 林金元[1] 杨立年 王慧[1] 戴惠学 胡颖雄 郑洪建 KUANG Huiyun;SUN Pingdong∗;LIN Jinyuan;YANG Linian;WANG Hui;DAI Huixue;HU Yingxiong;ZHENG Hongjian(Crop Breeding&Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Engineering Research Center of Specialty Maize,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201403,China;Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Nanjing Vegetable Science Research Institute,Nanjing 210042,China;CIMMYT-China Specialty Maize Research Center,Shanghai 201403,China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海特用玉米工程技术研究中心,上海市农业遗传育种重点实验室,上海201403 [2]上海市农业科学院,上海201403 [3]南京市蔬菜科学研究所,南京210042 [4]CIMMYT-中国特用玉米研究中心,上海201403

出　　处：《上海农业学报》2024年第2期24-30,共7页Acta Agriculturae Shanghai

基　　金：上海市科技兴农项目[沪农科推字(2022)第1-10号]。

摘　　要：为探讨玉米须多糖含量及体外生物活性,采用热水浸提-醇沉法提取3个品种(‘黑金699’‘金银308’和‘夏王’)玉米须多糖,并对其理化性质和体外生物活性进行对比。结果表明:‘黑金699’‘金银308’和‘夏王’3个品种玉米须多糖含量分别为(32.57±0.12)mg∕g、(96.39±0.76)mg∕g和(27.48±0.63)mg∕g。理化性质研究表明,3个品种玉米须多糖主要由阿拉伯糖、半乳糖和葡萄糖组成,且分子量大小分布不同。红外光谱分析表明,3个品种玉米须多糖均为β构型的酸性吡喃多糖。体外活性研究表明,3个品种玉米须多糖具有良好抗氧化、降血糖和抗肿瘤活性,且呈剂量依赖性,其中‘黑金699’玉米须多糖表现最为优异。综上,3个品种玉米须多糖具有较好的生物活性,该研究可为其在功能性食品、药品和化妆品中的应用提供依据。The polysaccharides were extracted from three varieties(‘Heijin 699’,‘Jinyin 308’,and‘Xiawang’)of corn silk by hot water extraction-alcohol sedimentation method,and the physicochemical properties and in vitro bioactivities of these polysaccharides were comparatively studied.The results showed that the contents of three polysaccharides(‘Heijin 699’,‘Jinyin 308’,and‘Xiawang’)were(32.57±0.12)mg∕g,(96.39±0.76)mg∕g and(27.48±0.63)mg∕g,respectively.The results of the physicochemical properties showed that the three polysaccharides mainly consisted of arabinose,galactose and glucose,and had different molecular weight distributions.Fourier transform-infrared spectra analysis showed that three polysaccharides were acidic pyran polysaccharides withβ-configuration.In vitro bioactivities results indicated that three polysaccharides possessed good antioxidant,hypoglycemic,and antitumor activities in a dose-dependent manner,among which polysaccharides in‘Heijin 699’showed the best performance.In conclusion,three polysaccharides have good biological activities,which provide an experimental basis for their application in functional food,pharmaceuticals,and cosmetics.

关 键 词：玉米须 多糖 理化性质 抗氧化 降血糖、抗肿瘤 

分 类 号：S513[农业科学—作物学] R285[医药卫生—中药学]