氢化镁颗粒对牙周致病菌具核梭杆菌的体外抗菌作用  

In vitro study on antibacterial effects of magnesium hydride particles against periodontitis-causing Fusobacterium nucleatum

作　　者：陈雨潇 饶定成 隋佰延 刘昕[1] Chen Yuxiao;Rao Dingcheng;Sui Baiyan;Liu Xin(Department of Dental Materials,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Institute of Stomatology,Shanghai 200011)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔材料科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《口腔材料器械杂志》2024年第2期82-87,共6页Chinese Journal of Dental Materials and Devices

基　　金：国家自然科学基金项目(编号:82271024);上海市科学技术委员会项目(编号:21DZ2291700);上海市重中之重研究中心项目(编号:2022ZZ01017);中国医学科学院医学与健康科技创新工程项目(编号:2019-I2M-5-037)。

摘　　要：目的评价微米氢化镁(MgH_(2))颗粒对牙周炎致病菌具核梭杆菌的体外抗菌作用,研究可能的抗菌机制。方法通过扫描电镜(SEM)和X射线衍射仪(XRD)对实验材料MgH_(2)颗粒的尺寸大小、表面形貌和晶相结构进行表征。使用电子顺磁共振技术(EPR)研究其释放行为,同时使用菌落计数实验(CFU)、溴化四甲基偶氮唑盐法(MTT)研究了MgH_(2)颗粒对具核梭杆菌的抗菌效应。结果MgH_(2)颗粒呈现为尺寸不均一的微米级球状颗粒,平均尺寸为10.52μm。XRD谱图具有属于MgH_(2)的(110)、(101)、(200)及(211)特征面的衍射信号。在菌落计数实验(CFU)实验中,当材料浓度达到1.5 mg/mL和2 mg/mL时有显著的抑菌效果,抗菌率分别为45.69%和79.24%。在MTT实验中,具核梭杆菌的活性随着MgH_(2)颗粒浓度的增加而逐渐降低,当MgH_(2)颗粒浓度达到2 mg/mL时,其活性接近于零。结论MgH_(2)颗粒对具核梭杆菌有着良好的抑菌效果,在临床应用方面具有良好的前景。Objective To evaluate the antibacterial effects of micron-sized magnesium hydride(MgH_(2))particles on periodontitis-causing Fusobacterium nucleatum and to explore the possible antibacterial mechanisms.Methods The size,surface morphology,and crystal phase structure of MgH_(2) particles were characterized by scanning electron microscopy(SEM)and X-ray diffraction(XRD).The release behavior of reactive oxygen radicals from MgH_(2) particles was studied by electron paramagnetic resonance(EPR)technology,and the antibacterial effects of MgH_(2) particles on Fusobacterium nucleatum were evaluated by colony counting assay(CFU)and tetrazolium bromide salt method(MTT).Results The MgH_(2) particles presented as micron-sized spherical particles of inhomogeneous size with an average size of 10.52μm.The XRD spectrum had diffraction signals of the(110),(101),(200),and(211)characteristic planes belonging to MgH_(2).In the CFU experiment,there was a significant antibacterial effect at the MgH_(2) concentration of 1.5 mg/mL and 2 mg/mL,with antibacterial rates of 45.69%and 79.24%,respectively.In the MTT assay,the viability of Fusobacterium nucleatum decreased gradually with the increase of MgH_(2) concentration,and its viability was close to zero when the concentration of MgH_(2) particles reached 2 mg/mL.Conclusion MgH_(2) particles have good antibacterial effects on Fusobacterium nucleatum,and show promising potential for clinical application.

关 键 词：氢化镁 抗菌作用 活性氧 具核梭杆菌 

分 类 号：R781.4[医药卫生—口腔医学]