基于PD-L1蛋白调控的五味子酚对肝细胞癌细胞增殖的影响  

Effects of Schisanhenol on Proliferation of Hepatocellular Carcinoma Cells Regulated by PD-L1 Protein

作　　者：韩旭 胡雪阳 王玉涵 黄蕾 李梓晴 李思颖 孙海明 王春梅[1] 李贺[1] 孙靖辉[1] 吴吉平 张志宏 HAN Xu;HU Xueyang;WANG Yuhan;HUANG Lei;LI Ziqing;LISiying;SUN Haiming;WANG Chunmei;LI He;SUN Jinghui;WU Jiping;ZHANG Zhihong(School of Pharmacy,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学药学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2024年第3期319-322,共4页Journal of Beihua University（Natural Science）

基　　金：国家自然科学基金青年基金项目(82204697);吉林省自然科学基金项目(YDZJ202201ZYTS275);吉林省教育厅科学技术研究项目(JJKH20230086KJ);北华大学研究生创新计划项目(2022030)。

摘　　要：目的探究五味子酚(Schisanhenol)对肝细胞癌细胞增殖及作用机制的影响。方法将HepG2细胞分为空白对照组和五味子酚3、10、30μmol/L 3个浓度组。应用Western Blot检测HepG2细胞PD-L1蛋白表达;通过免疫荧光试验进一步检测五味子酚对PD-L1蛋白表达的影响;通过集落试验观察HepG2细胞的增殖情况。结果HepG2细胞PD-L1蛋白表达结果显示,3、10、30μmol/L五味子酚均可显著抑制PD-L1蛋白表达(P<0.05或P<0.01),且抑制效果呈剂量依赖性;免疫荧光试验和集落试验结果显示,30μmol/L五味子酚对HepG2细胞增殖有显著的抑制作用(P<0.01)。结论五味子酚通过调节PD-L1蛋白表达抑制肝细胞癌细胞HepG2增殖。To investigate the effect of Schisanhenol on the proliferation of hepatocellular carcinoma cells and its related mechanism.[WTHZ]Method[WT][KG1]Hepatocellular carcinoma HepG2 cells were divided into blank control group and three Schisanhenol groups(3μmol/L,10μmol/L and 30μmol/L).The expressions of PD-L1 proteins in HepG2 cells were detected by Western Blot.Immunofluorescence assay was used to further detect the effect of Schisanhenol on PD-L1 protein.The proliferation of HepG2 cells were observed by cell colony assay.[WTHZ]Results[WT][KG1]The expression results of PD-L1 proteins in HepG2 cells showed that Schisanhenol with 3,10 and 30μmol/L could inhibit the expression of PD-L1 proteins(P<0.05 or P<0.01);The results of immunofluorescence assay and colony assay showed that Schisanhenol with 30μmol/L could obviously inhibit the proliferation of HepG2 cells(P<0.01).[WTHZ]Conclusion[WT][KG1]Schisanhenol inhibits the proliferation of liver cancer cell HepG2 through regulating PD-L1 protein.

关 键 词：五味子酚 肝细胞癌 PD-L1 增殖 

分 类 号：R9[医药卫生—药学] R735.7