消瘤藤乙酸乙酯提取物对结直肠癌细胞增殖、凋亡及Bax/Bcl-2/Caspase-3信号通路的影响  

Xiaoliuteng ethyl acetate extraction's impact on proliferation,apoptosis,and the Bax/Bcl-2/Caspase-3 signaling pathway in human colorectal cancer cells

作　　者：周欢思 李艳萍 金明静 赵湘培 赵昱倩 卢春花 ZHOU Huansi;LI Yanping;JIN Mingjing;ZHAO Xiangpei;ZHAO Yuqian;LU Chunhua(Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Guangxi Nanning 530000,China;Research Laboratory of Zhuang&Yao Medicine,Guangxi International Zhuang Medicine Hospital,Guangxi Nanning 530000,China;Medical Experimental Center,the First People's Hospital of Nanning,Guangxi Nanning 530021,China)

机构地区：[1]广西中医药大学附属国际壮医医院,广西南宁530000 [2]广西国际壮医医院壮瑶医药研究实验室,广西南宁530000 [3]南宁市第一人民医院医学实验中心,广西南宁530021

出　　处：《现代肿瘤医学》2024年第10期1767-1773,共7页Journal of Modern Oncology

基　　金：广西自然科学基金(编号:2018GXNSFDA050009);广西中医药大学博士启动基金(编号:2020BS034);广西中医药大学附属国际壮医医院人才引进科研启动基金(编号:GZ2021RC016)。

摘　　要：目的:研究消瘤藤乙酸乙酯提取物(XLTE)对人结直肠癌HCT116和HT29细胞增殖、凋亡及细胞周期的影响,并探索其与Bax/Bcl-2/Caspase-3信号通路的关系。方法:通过Incucyte S3活细胞动态分析系统监测XLTE作用下HCT116和HT29细胞形态变化并拟合48 h的IC50值,采用CCK-8实验、集落形成实验、细胞周期实验检测细胞增殖能力;通过Hoechst 33258染色、Rhodamine 123染色和Western blot检测细胞凋亡情况和Bax、Bcl-2、Caspase-3基因的表达。结果:XLTE可以抑制HCT116、HT29细胞增殖,48 h的IC50分别为567μg/mL和549μg/mL,XLTE作用下细胞周期主要阻滞在G0/G1期,并促进细胞凋亡,细胞染色质发生固缩或断裂,线粒体膜电位降低,Bax、Caspase-3基因的表达量增加,Bcl-2基因的表达量降低。结论:研究表明XLTE抑制人结直肠癌HCT116、HT29细胞增殖并促进细胞凋亡,与阻滞细胞周期在G0/G1期和激活Bax/Bcl-2/Caspase-3通路有关。Objective:To investigate the effects of Xiaoliuteng ethyl acetate extraction(XLTE)on human colorectal cancer cells(HCT116 and HT29),with a focus on its impact on cell proliferation,apoptosis,and the cell cycle,and to explore its relationship with Bax/Bcl-2/Caspase-3 signaling pathway.Methods:The morphological changes of HCT116 and HT29 cells under XLTE were monitored by Incucyte S3 live cell dynamic analysis system and IC 50 values of 48 h were fitted.The proliferation ability of HCT116 and HT29 cells was detected by CCK-8 assay,colony formation assay and cell cycle assay.The proapoptotic ability of HCT116 and HT29 cells was detected by Hoechst 33258 staining,Rhodamine 123 staining and the expressions of Bax,Bcl-2 and Caspase-3 proteins were detected by Western blot.Results:XLTE exhibited significant inhibitory effects on the proliferation of HCT116 and HT29 cells,with 48 h's IC 50 values of 567μg/mL and 549μg/mL,respectively.In addition,XLTE was found to arrest the cell cycle in the G 0/G 1 phase,promote apoptosis,induce chromatin contraction and breakage,and reduce mitochondrial membrane potential.Furthermore,XLTE was found to increase the expression of Bax and Caspase-3 protein,while decreasing the expression of Bcl-2 protein in the cells.Conclusion:XLTE was found to inhibit proliferation and promote apoptosis in HCT116 and HT29 cells of human colorectal cancer,which was related to the blocking of the cell cycle in the G 0/G 1 phase and the activation of the Bax/Bcl-2/Caspase-3 pathway.

关 键 词：消瘤藤 结直肠癌 细胞周期 线粒体膜电位 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤]