SNORA73B高表达对喉鳞癌干细胞样特征的影响  

The effect of high expression of SNORA73B on stem cell-like characteristics in laryngeal squamous cell carcinoma

作　　者：王晶田[1] 赵岩[1] 吴干勋[1] 刘胜辉[1] 兰利利[1] 胡国斌 徐玉茹[1] 郝凯凯 沈素朋[2] WANG Jingtian;ZHAO Yan;WU Ganxun;LIU Shenghui;LAN Lili;HU Guobin;XU Yuru;HAO Kaikai;SHEN Supeng(Otorhinolaryngology Head and Neck Surgery,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China;Biological Specimen Library,Hebei Provincial Cancer Institute,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院耳鼻喉头颈外科,河北石家庄050011 [2]河北医科大学第四医院、河北省肿瘤研究所生物标本库,河北石家庄050011

出　　处：《现代肿瘤医学》2024年第10期1799-1804,共6页Journal of Modern Oncology

基　　金：河北省重点研发计划项目(编号:22377736D,22377721D);河北省自然科学基金项目(编号:H2022206326)。

摘　　要：目的:以小核仁RNA73B(small nucleolar RNA 73B,SNORA73B)在喉鳞癌(laryngeal squamous cell carcinoma, LSCC)中高表达及其临床意义为切入点,探讨SNORA73B可能通过维持干细胞特征,从而促进LSCC细胞的增殖、迁移和侵袭。方法:qPCR检测LSCC组织和细胞中SNORA73B的表达情况;体外培养细胞,MTS、划痕愈合、Transwell实验分别检测SNORA73B敲低或过表达对细胞增殖、迁移和侵袭的影响;肿瘤细胞球形成实验,检测SNORA73B对LSCC细胞干性样特征的影响。结果:qPCR检测结果显示,SNORA73B在LSCC组织和细胞中表达显著高于相应癌旁组织和细胞对照组(均P<0.05),且与患者病理分级、淋巴结转移和不良预后有关(P<0.05)。过表达SNORA73B的Tu177细胞增殖、划痕愈合、迁移和侵袭能力明显增强(均P<0.05);而干扰SNORA73B后细胞增殖、划痕愈合、迁移和侵袭能力明显降低(均P<0.05)。在诱导LSCC干细胞样特性的喉癌球细胞形成后,肿瘤干细胞标志物OCT4、SOX2蛋白表达明显升高(P<0.05),SNORA73B表达显著升高(P<0.05);干扰SNORA73B,细胞球形成数明显减少(P<0.05),OCT4和SOX2 mRNA(P<0.05)和蛋白表达明显降低。结论:SNORA73B高表达可能与LSCC的发生和恶性进展有关,可能介导LSCC细胞的干性样特征促进肿瘤细胞的增殖和迁移。Objective:To investigate the potential role of small nucleolar RNA 73B(SNORA73B)in laryngeal squamous cell carcinoma(LSCC)by examining its high expression and clinical significance,to explore how SNORA73B may contribute to the proliferation,migration,and invasion of LSCC cells by maintaining stem cell characteristics.Methods:Quantitative PCR(qPCR)was employed to measure the expression level of SNORA73B in LSCC tissues and cells.Furthermore,in vitro cultured cells were utilized for MTS assays,scratch healing assays,and Transwell experiments to assess the impact of SNORA73B knockdown or overexpression on cell proliferation,migration,and invasion.Additionally,the role of SNORA73B in influencing the stem cell-like characteristics of LSCC cells was evaluated using a tumor cell spheroidization assay.Results:The results obtained from qPCR analysis demonstrated a substantial increase in the expression of SNORA73B in both LSCC tissues and cells compared to the corresponding adjacent cancer tissues and control group(P<0.05).This elevated expression was found to be associated with pathological grading,lymph node metastasis,and poor prognosis in patients(P<0.05).Moreover,overexpression of SNORA73B in Tu177 cells notably enhanced their proliferation,scratch healing,migration,and invasion capabilities(P<0.05).Conversely,interference with SNORA73B led to a significant reduction in these cellular functions(P<0.05).To further investigate the impact of SNORA73B on the development of LSCC stem cell-like characteristics,laryngeal cancer spheroid cells were induced(P<0.05).The induction resulted in a notable increase in the expression of tumor stem cell markers OCT4 and SOX2 proteins(P<0.05).Additionally,SNORA73B expression was significantly upregulated(P<0.05).However,interference with SNORA73B led to a significant decrease in the number of cell spheroids(P<0.05)as well as reduced mRNA and protein expressions of OCT4 and SOX2.Conclusion:The high expression of SNORA73B appears to be associated with the development and malignant progr

关 键 词：小核仁RNA SNORA73B 喉鳞状细胞癌 预后 肿瘤干细胞 

分 类 号：R739.65[医药卫生—肿瘤]