胃癌中端粒相关基因亚型鉴定、预后模型构建以及免疫细胞浸润分析  

Identification of telomere related gene subtypes,construction of prognostic model and analysis of immune cell infiltration in gastric cancer

作　　者：田文集 张德奎[2] TIAN Wen-ji;ZHANG De-kui(The Second Clinical Medical College of Lanzhou University,Lanzhou 730030,China;Department of Gastroenterology,Lanzhou University Second Hospital,Lanzhou 730030,China)

机构地区：[1]兰州大学第二临床医学院,730030 [2]兰州大学第二医院消化内科,730030

出　　处：《现代消化及介入诊疗》2024年第2期163-169,175,共8页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：联合科研基金重大项目(23JRRA1501);甘肃省自然科学基金(22JR5RA960);兰州大学医学创新发展项目(lzuyxcx-2022-181)。

摘　　要：目的本研究旨在基于对端粒相关基因(Telomere related genes,TRG)表达数据的综合分析,探讨预后分子特征以预测胃癌(GC)的预后及与免疫细胞浸润的关系。方法联合TCGA与GEO数据库对胃癌端粒相关基因进行生存分析及预后相关性分析。通过无监督聚类确定两个端粒相关基因簇,依据Lasso回归及多因素cox回归分析构建预后模型,结合临床性状构建列线图预测患者生存期,同时进行风险差异分析,运用ROC曲线评估模型预测准确性,对样本进行免疫细胞浸润分析。结果构建了包含HEYL、SPC25、SRPX2、PDK4、LOXL4、SOX15、SLC39A4、MAGEA3、SHISA2、DEFB1、SLC27A2和C1QTNF5等12个基因的预后模型来评估胃癌患者的预后,构建了整合风险特征的列线图来预测胃癌患者的1年、3年和5年总生存期(OS),高风险组的生存率显著低于低风险组(P<0.05),不同风险组的免疫细胞亚型比例不同。结论这项研究鉴定了胃癌中TRG衍生的分子亚型,并开发了一种新的预后评分模型,突出了TRG在胃癌预后和免疫治疗中的潜在价值。Objective The aim of this study is to investigate the prognostic molecular features to predict the prognosis of gastric cancer(GC)and its relationship with immune cell infiltration based on comprehensive analysis of telomere-related gene(TRG)expression data.Methods The survival and prognostic correlation of GC telomere-related gene were analyzed by combining TCGA and GEO database.Two telomere related gene clusters were identified by unsupervised clustering,a prognostic model was constructed based on Lasso regression and multi-factor cox regression analysis,and a nomogram was constructed combined with clinical traits to predict the survival of patients.Meanwhile,risk difference analysis was performed,ROC curve was used to evaluate the prediction accuracy of the model,and immune cell infiltration analysis was performed on the samples.Results A prognostic model containing 12 genes,including HEYL,SPC25,SRPX2,PDK4,LOXL4,SOX15,SLC39A4,MAGEA3,SHISA2,DEFB1,SLC27A2 and C1QTNF5,was constructed to evaluate the prognosis of GC patients.An integrated risk profile was constructed to predict 1-year,3-year,and 5-year overall survival(OS)of GC patients.The survival rate in the high-risk group was significantly lower than that in the low-risk group(P<0.05),and the proportion of immune cell subtypes in different risk groups was different.Conclusion This study identifies a TRG-derived molecular subtype in GC and develops a new prognostic scoring model,highlighting the potential value of TRG in GC prognosis and immunotherapy.

关 键 词：端粒相关基因 胃癌 免疫浸润 预后模型 

分 类 号：R735.2[医药卫生—肿瘤] R571[医药卫生—临床医学]