Cullin7在胃癌中的表达及相关性分析  

Analysis on the expression and correlation of Cullin7 in gastric cancer

作　　者：刘业锋 李飞[1] 谢伟 王星 LIU Yefeng;LI Fei;XIE Wei;WANG Xing(Department of General Surgery,The People′s Hospital of Jurong,Jurong 212400,China;Department of Pharmacology,School of Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]句容市人民医院普外科,江苏句容212400 [2]中国药科大学药学院药理系,江苏南京211198

出　　处：《药学研究》2024年第4期327-332,共6页Journal of Pharmaceutical Research

基　　金：江苏省基础研究计划(自然科学基金)-青年基金项目(No.BK20180574)。

摘　　要：目的探讨泛素连接酶CUL7蛋白抗体(Cullin7)在胃癌中的表达及其对胃癌发生发展的作用。方法自癌症基因组图谱(TCGA)数据库下载胃癌患者mRNA水平数据,并进行生存状况分析;并收集了60例接受手术治疗胃癌患者的胃癌和癌旁组织样本,免疫组化分析Cullin7的表达水平,并评估Cullin7表达水平与胃癌患者临床病理特征之间的相关性;同时通过在人胃癌HGC-27细胞系中过表达或敲除Cullin7基因,利用四甲基偶氮唑盐(MTT)、集落形成、划痕、Transwell迁移和Matrigel侵袭实验研究Cullin7对胃癌细胞增殖、迁移和侵袭的影响。结果Cullin7在胃癌组织中高表达,且Cullin7高表达的患者总生存期、首次进展和进展后生存时间更短,同时Cullin7的表达与胃癌患者分化程度、神经侵袭、血管侵袭、浸润深度、淋巴结转移、临床分期相关,差异有统计学意义,而Cullin7在胃癌细胞中的过表达促进细胞增殖、迁移和侵袭,而用短发夹RNA(shRNA)敲低Cullin7可以抑制胃癌细胞的增殖、迁移和侵袭,差异有统计学意义。结论Cullin7的上调可能是促进胃癌发生和发展的重要因素,可作为胃癌诊断、预后新的生物标志物以及新的治疗靶点。Objective To investigate the expression of Cullin7 in gastric cancer and its role in the genesis and development of gastric cancer.Methods Download the mRNA level data of gastric cancer patients from The Cancer Genome Atlas(TCGA)database,and analyze the survival status;The expression level of Cullin 7 in gastric cancer and adjacent tissues of 60 patients with gastric cancer undergoing surgical treatment was analyzed by immunohistochemistry,and the correlation between the expression level of Cullin 7 and the clinicopathological characteristics of gastric cancer patients was evaluated.At the same time,through overexpression or knockout of Cullin7 gene in human gastric cancer HGC-27 cell line,the effects of Cullin7 on the proliferation,migration and invasion of gastric cancer cells were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),colony formation,wound healing,Transwell migration and Matrigel invasion experiments.Results Cullin7 is highly expressed in gastric cancer,and patients with high expression of Cullin7 have shorter overall survival,and shorter first progression and post progression survival times.At the same time,the expression of Cullin7 is significantly related to the pathologic differentiation,nerve invasion,vascular invasion,depth of invasion,lymph node metastasis and clinical stage of gastric cancer patients.The ectopic expression of Cullin7 in gastric cancer cells significantly promotes cell proliferation,migration and invasion,while knocking down Cullin7 with short hairpin RNA(shRNA)can inhibit the proliferation,migration and invasion of gastric cancer cells.Conclusion Upregulation of Cullin7 may be an important factor promoting the occurrence and development of gastric cancer,and can be used as a new biomarker for gastric cancer diagnosis and prognosis as well as a new therapeutic target.

关 键 词：胃癌 泛素连接酶CUL7蛋白抗体 预后 临床病理特征 增殖 迁移 侵袭 

分 类 号：R730.231[医药卫生—肿瘤]