抗氧化营养素与妊娠并发症的孟德尔随机化研究  

Association between antioxidant nutrients and pregnancy complications:a Mendelian randomization study

作　　者：王爽 谭茜蔚 令垚 黄瑞贤 马勇 孔丹莉 丁元林 WANG Shuang;TAN Xiwei;LING Yao;HUANG Ruixian;MA Yong;KONG Danli;DING Yuanlin(School of Public Health,Guangdong Medical University,Dongguan,Guangdong 523808,China)

机构地区：[1]广东医科大学公共卫生学院,广东东莞523808

出　　处：《预防医学》2024年第4期345-349,共5页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：广东医科大学学科建设项目(4SG21276P);广东省基础与应用基础研究基金区域联合基金项目(2020B1515120021)。

摘　　要：目的探讨抗氧化营养素与妊娠并发症的因果关系,为妊娠并发症防治提供参考。方法通过全基因组关联研究(GWAS)目录和芬兰数据库获得维生素A、维生素C、维生素E、硒、锌、铜和铁7种抗氧化营养素,以及妊娠糖尿病、先兆子痫、自然流产和早产4种妊娠并发症的GWAS数据,筛选与7种抗氧化营养素存在强相关的27个单核苷酸多态性位点(SNP)作为工具变量。采用逆方差加权法(IVW),以7种抗氧化营养素为暴露因素,以4种妊娠并发症为结局变量,进行孟德尔随机化(MR)分析;采用Cochran Q检验评估异质性,采用MR-Egger回归法和MR-PRESSO检验水平多效性。结果Cochran Q检验显示,维生素C与妊娠糖尿病、先兆子痫、早产的MR结果存在异质性,维生素E、铁与妊娠糖尿病的MR结果存在异质性(均P<0.05),采用随机效应模型;其他结果不存在异质性(均P>0.05),采用固定效应模型。MR分析结果显示,7种抗氧化营养素与4种妊娠并发症发生风险不存在因果关联(均P>0.05)。MREgger回归法、MR-PRESSO检验表明工具变量间不存在水平多效性(均P>0.05)。结论本研究未发现遗传学预测的抗氧化营养素与妊娠并发症存在关联。Objective To investigate the causal relationship between antioxidant nutrients and pregnancy complications,so as to provide the reference for the prevention and treatment of pregnancy complications.Methods Data of seven an-tioxidant nutrients including vitamin A,vitamin C,vitamin E,selenium,zinc,copper and iron were collected from ge-nome-wide association study(GWAS)Catalog database,and data of four pregnancy complications including gestational di-abetes mellitus,pre-eclampsia,spontaneous abortion and preterm birth were collected from the Finland database.Single nucleotide polymorphism(SNP)data were collected,and 27 SNPS strongly correlated with seven antioxidant nutrients were selected as instrumental variables.Mendelian randomization(MR)analyses were performed using the inverse-vari-ance weighted(IVW)method with seven antioxidant nutrients as exposures factors and four pregnancy complications as outcome variables.The heterogeneity was assessed using the Cochran's Q test,the horizontal pleiotropy was assessed us-ing the MR-PRESSO test and MR-Egger regression,and the robustness of the results was verified with the leave-one-out.Results Cochran's Q test showed heterogeneity of MR results between vitamin C and gestational diabetes mellitus,preeclampsia and preterm birth,between vitamin E and iron and gestational diabetes(all P<0.05),and a random effect model was employed.There was no heterogeneity in other results(all P>0.05),and a fixed effect model was employed.MR analysis results showed that there was no causal association between seven antioxidant nutrients and the risk of four pregnancy complications(all P>0.05).MR-PRESSO test and the MR-Egger regression identified no horizontal plei-otropy of instrumental variables(both P>0.05).Conclusion This study did not find genetically predicted associations of antioxidant nutrients with pregnancy complications.

关 键 词：抗氧化营养素 妊娠并发症 孟德尔随机化 

分 类 号：R714.25[医药卫生—妇产科学]