基于高通量测序分析中草药对发酵泡菜亚硝酸盐含量的影响及机制  

Analysis of Effect and Mechanism of Chinese Herbal Medicine on Nitrite Content in Fermented Pickles Based on High-Throughput Sequencing

作　　者：燕平梅[1] 张聪聪 贾晶晶 赵虎威 YAN Ping-mei;ZHANG Cong-cong;JIA Jing-jing;ZHAO Hu-wei(Taiyuan Normal University,Taiyuan 030619,China)

机构地区：[1]太原师范学院,太原030619

出　　处：《中国调味品》2024年第5期59-64,共6页China Condiment

基　　金：国家自然基金委员会面上项目(40871131,31171743);山西省自然基金面上项目(202303021211119)。

摘　　要：为了探究金银花、黄柏两种中草药对发酵泡菜亚硝盐含量的影响及机制,基于高通量测序技术分析泡菜微生物群落结构,采用分光光度法分析泡菜中亚硝盐含量。研究结果表明,分别添加5%金银花和5%黄柏的泡菜中亚硝酸盐含量(8.02,5.90 mg/kg)均显著低于对照组(12.87 mg/kg)(P<0.05)。对泡菜样品进行高通量测序,共得到202个可操作分类单元(OTU),分属于11个门、129个属、165个种。添加5%的金银花和5%黄柏发酵10 d的泡菜样品中微生物的Chao 1指数显著低于对照组(P<0.05)。Proteobacteria(变形菌门)是各个样品中的优势菌门,相对含量高达90%。不同中草药处理的微生物在属水平上的优势微生物相对含量相差很大,对照组、添加金银花的发酵泡菜组、添加黄柏的发酵泡菜组中Pseudomonas(假单胞菌属)的相对含量分别为52%、1%、1%;Serratia(沙雷氏菌属)的相对含量分别为15%、0%、1%;Achromobacter(无色杆菌属)的相对含量分别为7%、1%、0%。实验结果表明金银花和黄柏对发酵白菜中亚硝酸盐形成的抑制作用是由于其抑制了肠杆菌科(Serratia、Achromobacter)和Pseudomonas(假单胞菌属)微生物的生长繁殖。In order to explore the effect and mechanism of two Chinese herbal medicines,namely honeysuckle and phellodendron,on nitrite content in fermented pickles,the microbial community structure of pickles is analyzed based on high-throughput sequencing technology,and spectrophotometry is used to analyze the nitrite content in pickles.The results show that the nitrite content of pickles added with 5%honeysuckle and 5%phellodendron(8.02,5.90 mg/kg)is significantly lower than that of the control group(12.87 mg/kg)(P<0.05).Through high-throughput sequencing of pickle samples,a total of 202 operational taxonomic units(OTU)are obtained,belonging to 11 phyla,129 genera and 165 species.The Chao 1 index of microorganism in pickle samples added with 5%honeysuckle and 5%phellodendron fermented for 10 d is significantly lower than that of the control group(P<0.05).Proteobacteria is the dominant phylum in each sample,with the relative content of 90%.The relative content of dominant microorganisms in genus level of microorganisms treated with different Chinese herbal medicines is quite different.The relative content of Pseudomonas in the control group,the pickle sample group added with honeysuckle and the pickle sample group added with phellodendron is 52%,1%1%respectively.The relative content of Serratia is 15%,0%,1%respectively.The relative content of Achromobacter is 7%,1%,0%respectively.The experimental results show that the inhibitory effect of honeysuckle and phellodendron on the formation of nitrite in fermented Chinese cabbage is due to their inhibition on the growth and reproduction of microorganisms of Enterobacteriaceae(Serratia,Achromobacter)and Pseudomonas.

关 键 词：高通量测序 发酵泡菜 中草药 亚硝酸盐 微生物多样性 

分 类 号：TS255.54[轻工技术与工程—农产品加工及贮藏工程]