入口电离-高分辨质谱法分析人参皂苷同分异构体  

Analysis of Ginsenoside Isomers by Inlet Ionization-High Resolution Mass Spectrometry

作　　者：郭云龙 迟鸿悦 王洋 郑飞 戴雨霖 黄鑫 越皓 刘淑莹 GUO Yun-long;CHI Hong-yue;WANG Yang;ZHENG Fei;DAI Yu-lin;HUANG Xin;YUE Hao;LIU Shu-ying(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China;Changchun Center of Mass Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]长春中医药大学,吉林省人参科学研究院,吉林长春130117 [2]中国科学院长春应用化学研究所,长春质谱中心,吉林长春130022

出　　处：《质谱学报》2024年第3期396-400,I0004,共6页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(81903778);吉林省道地药材健康产品开发工程研究中心(吉发改高技[2017]1057)。

摘　　要：本文建立了入口电离-高分辨质谱法快速区分人参皂苷同分异构体,通过正、负离子模式及最佳入口温度,对人参皂苷同分异构体进行敞开式质谱分析。结果表明,在正、负离子全扫描模式及最佳实验温度下,根据人参皂苷同分异构体所形成的聚体数目不同,形成的离子峰类型不同,可实现人参皂苷同分异构体的区分。该方法简单、快速,仅需简单的样品前处理,即可区分人参皂苷同分异构体。The ginsenoside isomers were analyzed and distinguished by using inlet ionization-high resolution mass spectrometry technology under positive and negative ion modes with optimum inlet temperatures.The results showed that under the full scanning mode of positive and negative ion modes with the optimum experimental temperature,different types of ions were formed according to the characteristics of different numbers of polymers formed by isomeric monomers of ginsenoside,and the identification of ginsenoside isomers was realized.For the identification of ginsenoside CK and Rh2,there was no obvious difference in their MS spectra under positive ion mode.However,under negative ion mode with the inlet temperature of 200℃,ginsenoside CK tended to form polymers of[M+Cl]^(-),Rh_(2)tended to form polymers of[M+COOH]^(-),and the number of polymers of CK was significantly higher than that of Rh2.For the identification of ginsenoside Rh_(2) and Rc,under positive ion mode with the inlet temperature of 200℃,ginsenoside Rh_(2) and Rc could produce polymers of[M+Na]+,but the polymers peak intensity of Rh_(2) was significantly higher than that of Rc.Under negative ion mode with the inlet temperature of 200℃,Rb_(2) tended to form polymers of[M+Cl]^(-),and Rc tended to form polymers of[M+COOH]^(-).For the identification of ginsenoside Rg1 and Rf,there was no obvious difference in their MS spectra under the positive ion mode.Under negative ion mode with the inlet temperature of 200℃,Rg1 and Rf tended to form polymers of[M+Cl]^(-)and[M−H]^(-),respectively.In this study,the three pairs of isomers in ginsenosides were basically identified by using the full MS scanning method and the control of the mass spectrometry inlet temperature.The experimental method is simple,fast,and has the characteristics of low consumption.This study confirms that the entrance ionization mass spectrometry technique can provide a theoretical reference for the rapid discrimination and identification of ginsenoside isomers.

关 键 词：入口电离 高分辨质谱 人参皂苷 同分异构体 

分 类 号：O657.63[理学—分析化学]