不同生长阶段中国花鲈肠道和环境微生物群落分析  

Structural differences in intestinal microbiota of Chinese sea bass(Lateolabrax maculatus)in different growth stages

作　　者：梁祖銮 赵吉臣 廖敏泽 孙成波[1,3] LIANG Zuluan;ZHAO Jichen;LIAO Minze;SUN Chengbo(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Key Laboratory of Pathogenic Biology and Epidemiology of Aquatic Economic Animals in Guangdong Province,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省农业科学院动物科学研究所广东省畜禽育种与营养研究重点实验室,农业农村部华南动物营养与饲料重点实验室,广东广州510640 [3]广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088

出　　处：《大连海洋大学学报》2024年第2期215-224,共10页Journal of Dalian Ocean University

基　　金：广东省重点领域研发计划项目(2020B0202010009);广东省普通高等学校创新团队项目(2021KCXTD026)。

摘　　要：为探究中国花鲈(Lateolabrax maculatus)不同生长阶段肠道和养殖环境微生物组成,选择3月龄(小规格组,体质量为79.89 g±14.97 g)、6月龄(中规格组,体质量为212.67 g±30.32 g)和9月龄(大规格组,体质量为427.56 g±69.56 g)3个不同生长阶段的中国花鲈,采集其肠道微生物和环境微生物(养殖水和池塘底泥)进行16S rDNA基因测序数据分析。结果表明:不同月龄的中国花鲈肠道微生物与环境微生物群落结构有较大差异,而不同生长阶段的环境微生物群落结构则无显著性差异(P>0.05);环境微生物中双色藻属(Cyanobium_PCC-6307)作为差异物种,表现出与硝酸盐氮呈负相关;肠道微生物分析显示,门水平上,3月龄和6月龄中国花鲈肠道中厚壁菌门(Firmicutes)丰度低于9月龄中国花鲈,属水平上,3月龄和6月龄中国花鲈肠道中富集的可促进糖利用的鲸杆菌属(Cetobacterium)丰度高于9月龄中国花鲈,罗姆布茨菌属(Romboutsia)肠道微生物差异贡献度最高。研究表明,不同养殖阶段的环境微生物群落结构无显著性差异,不同月龄中国花鲈间的肠道微生物群落结构有显著性差异,中国花鲈肠道微生物群落结构受养殖环境的影响。In order to investigate the microbial composition of intestine and aquaculture environment of Chinese sea bass(Lateolabrax maculatus)at different growth stages,16S rDNA gene sequence data were analyzed for microbiota in intestine of 3-month-old with body weight of(79.89±14.97)g(small size group),6-month-old with body weight of(212.67±30.32)g(middle size group),and 9-month-old with body weight of(427.56±69.56)g(large size group)Chinese sea bass and aquaculture water and pond sediments.The results showed that there were significant differences in the structure of intestinal microbial and environmental microbial communities in Chinese sea bass at different ages,while there was no significant difference in environmental microorganisms community structure at different growth stages(P>0.05).Cyanobium_PCC-6307 as a differential species in environmental microorganisms showed a negatively correlation with nitrate nitrogen levels.Analysis of intestinal microbiota revealed that the abundance of Firmicutes was lower in the intestines of 3-month-old and 6-month-old Chinese sea bass than that in 9-month-old Chinese sea bass at the phylum level.At the genus level,there was higher abundance of Cetobacterium promoting sugar utilization in the intestines of 3-month-old and 6-month-old Chinese sea bass than that in 9-month-old Chinese sea bass.There was the maximal differences contribution in Romboutsia at the genus level.The findings indicated that there is no significant difference in the microbial community structure of the environment at different breeding stages,but there was significant difference in the intestinal microbial community structure among Chinese sea bass at different months of age,the intestinal microbiota of Chinese sea bass was influenced by aquaculture environmental.

关 键 词：中国花鲈 肠道微生物 环境微生物 池塘底泥 

分 类 号：S917.1[农业科学—水产科学]