瑞香狼毒茎中黄酮提取工艺及抑菌活性研究  

Study on the Extraction Technology and Antibacterial Activity of Flavonoids from the Stem of Stellera Chamaejasme

作　　者：李彪 巩红冬 郭淑青 LI Biao;GONG Hongdong;GUO Shuqing(Department of Chemistry and Life Sciences,Gansu Minzu Normal University,Hezuo Gansu 747000)

机构地区：[1]甘肃民族师范学院化学与生命科学系,甘肃合作747000

出　　处：《甘肃高师学报》2024年第2期18-22,共5页Journal of Gansu Normal Colleges

基　　金：甘肃民族师范学院2017年校长基金项目“瑞香狼毒花多糖提取工艺优化及其抑菌活性研究”(17-09);2023年国家民委高等教育教学改革研究项目“基于创新型人才培养的植物生物学‘四位一体’实践教学体系的探索与实践”(23151)。

摘　　要：为合理开发利用瑞香狼毒茎中的黄酮,文章运用正交实验研究瑞香狼毒茎中黄酮的最优提取工艺,并分析瑞香狼毒茎黄酮对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的作用.结果表明:瑞香狼毒茎黄酮提取的最佳条件为温度70℃,乙醇浓度50%,料液比1∶20,回流时间保持2.5 h,提取率高达23.34%;瑞香狼毒茎黄酮对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌均有抑制作用,其对金黄色葡萄球菌和枯草芽孢杆菌的最小抑制浓度都为12.5 mg/mL,对大肠杆菌的最小抑制浓度为6.25 mg/mL.In order to rationally develop and utilize the flavonoids in the stem of Stellera chamaejasme,the optimum extraction process of flavonoids from the stem of Stellera chamaejasme was studied by orthogonal design,the effects of flavonoids from the stem of Stellera chamaejasme on Staphylococcus aureus,Bacillus subtilis and Escherichia coli were also analysed.The results showed that the optimum conditions for the extraction of flavonoids from the stems of Stellera chamaejasme were as follows:temperature 70 ℃,ethanol concentration50%,solid-liquid ratio 1 ∶ 20,reflux time 2.5 h,and the extraction rate was as high as 23.34%.The flavonoids from the stem of Stellera chamaejasme had inhibitory effects on Escherichia coli,Bacillus subtilis and Staphylococcus aureus.The minimum inhibitory concentration for both Staphylococcus aureus and Bacillus subtilis was 12.5 mg/m L,the minimum inhibitory concentration for Escherichia coli was 6.25 mg/m L.

关 键 词：瑞香狼毒 黄酮 抑菌活性 最小抑制浓度 

分 类 号：R284[医药卫生—中药学]