乳铁蛋白的重组表达系统及法规管理现状概述  

Overview of recombinant expression systems and regulation status of lactoferrin

作　　者：吴晓文 张长太 李佳佳 凡孝菊 周景文 WU Xiaowen;ZHANG Changtai;LI Jiajia;FAN Xiaoju;ZHOU Jingwen(Jiangsu Trautec Medical Technology Co.Ltd.,Changzhou 213000,China;Science Center for Future Foods,Jiangnan University,Wuxi 214000,China)

机构地区：[1]江苏创健医疗科技股份有限公司,江苏常州213000 [2]江南大学未来食品科学中心,江苏无锡214000

出　　处：《食品与发酵工业》2024年第9期321-329,共9页Food and Fermentation Industries

基　　金：江苏省自然科学基金前沿引领技术基础研究专项(BK20202002)。

摘　　要：乳铁蛋白是一种铁结合糖蛋白,广泛存在于哺乳动物的乳汁和黏膜分泌物当中。乳铁蛋白具有广谱抑菌、抗病毒、免疫调节等生理功能,在婴幼儿配方乳粉、营养补充剂、化妆品等领域都有应用。近年来,随着基因工程技术的不断发展,来自不同物种的乳铁蛋白在常见的原核体系、真核体系与转基因哺乳动物中均得到了成功表达,重组产物的表达量及其与天然乳铁蛋白的结构相似度也在不断提升。该文对乳铁蛋白的生物学活性、重组表达系统以及目前的法规管理情况进行综述,并讨论了重组乳铁蛋白的研究难点和未来发展方向。Lactoferrin is an iron-binding glycoprotein widely found in mammal milk and mucosal secretions.Lactoferrin has broad-spectrum antibacterial,antiviral,immunomodulatory,and other physiological functions,and is used in various fields,such as infant formula milk powder,nutritional supplements,and cosmetics.In recent years,with the continuous development of genetic engineering technology,lactoferrin from different species has been successfully expressed in common prokaryotic expression systems,eukaryotic expression systems,and transgenic mammals.Meanwhile,the expression of recombinant products and their structural similarity to natural lactoferrin have been increasing.This report reviewed the biological activity,recombinant expression systems,and current regulations of lactoferrin.Furthermore,the challenges and future development directions of recombinant lactoferrin were discussed.

关 键 词：乳铁蛋白 生理活性 重组表达系统 法规 应用 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]