Wireless electrical stimulation generated by piezoelectric nanomaterial promotes the dental pulp regeneration via regulating mitochondrial Ca^(2+)/PKA signaling pathway  

作　　者：Jianmao Zheng Jiyuan Zuo Cairong Xiao Qining Guo Xiaobin Fu Chengyun Ning Peng Yu 

机构地区：[1]Guangdong Provincial Key Laboratory of Stomatology,Hospital of Stomatology,Sun Yat-Sen University,Guangzhou 510080,China [2]School of Materials Science and Engineering,National Engineering Research Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou 510641,China

出　　处：《Journal of Materials Science & Technology》2024年第1期24-34,共11页材料科学技术（英文版）

基　　金：supported by the Guangdong Basic and Applied Basic Research Foundation(Nos.2022A1515011266,2019A1515011289);National Natural Science Foundation of China(Nos.52072127,81700950);Medical Scientific Research Foundation of Guangdong Province of China(A2022322).

摘　　要：The induction of dental pulp stem cells(DPSCs)into odontogenic differentiation is a cutting-edge method of dental pulp regeneration treatment.However,it remains a challenge to develop biomaterials and ther-apies that can induce odontogenic differentiation.Here,we propose a wireless electrical stimulation strat-egy to induce DPSCs odontogenic differentiation via K_(0.5)Na_(0.5)NbO_(3)piezoelectric nanoparticles(KNN)and polarized KNN(PKNN),which can be endocytosed by DPSCs.In vitro,several odontogenic differentiation indexes were also increased in DPSCs treated with KNN and PKNN,and the increase was more obvious in the PKNN group.Intracellular wireless electrical field promoted mitochondrial calcium concentration via mitochondrial calcium unidirectional transporter(MCU),increased the production of adenosine triphos-phate(ATP),and induced odontogenic differentiation through the activation of the cAMP-PKA signaling pathway.In vivo,dental pulp-like tissue was induced by electrical stimulation wirelessly with KNN and PKNN,which was more clinically friendly compared with the wired device,and the induction was more obvious in the PKNN group consistent with in vitro experiments.In conclusion,this work demonstrates the potential of PKNN as an effective stimulus that can induce odontogenic differentiation of DPSCs and be applied to dental pulp regeneration treatment.

关 键 词：Piezoelectric nanoparticles Dental pulp stem cells Odontogenic differentiation 

分 类 号：R78[医药卫生—口腔医学]