高效液相色谱法同时测定补肾健骨口服液中4种成分的含量  

Simultaneous Determination of Four Components in Bushen Jiangu Oral Liquid(补肾健骨口服液)By HPLC

作　　者：吴勇梅 卿丽婷 谢鲜丽 梁志敏 范卫锋 覃乐瑶 唐琳 谢洁兰 李耀华[2] 韦玉燕 WU Yongmei;QING Liting;XIE Xian;LIANG Zhimin;FAN Weifeng;QIN Leyao;TANG Lin;XIE Jielan;LI Yaohua;WEI Yuyan(Yulin Orthopaedic Hospital of Integrated Traditional Chinese and Western Medicine,Yulin Guangxi 537000,China;Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China)

机构地区：[1]玉林市中西医结合骨科医院,广西玉林537000 [2]广西中医药大学,广西南宁530200

出　　处：《中医药导报》2024年第4期57-60,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西壮族自治区中医药管理局中药壮瑶药医院制剂质量提升项目(GZZJ202034);玉林市科学研究与技术开发计划项目(玉市科20203220)。

摘　　要：目的:建立高效液相色谱(HPLC)法同时测定补肾健骨口服液中没食子酸、二苯乙烯苷、大黄素-8-O-β-D-葡萄糖苷、大黄素等4种成分含量的方法。方法:采用HPLC法,色谱柱为Shim-pack GIST C_(18)(250mm×4.6mm,5μm);流动相为乙腈-0.1%磷酸溶液,进行梯度洗脱;柱温为30℃;进样量为10μL;检测波长为285 nm。结果:4种成分线性关系良好,没食子酸、二苯乙烯苷、大黄素-8-O-β-D-葡萄糖苷、大黄素的线性质量范围分别是0.036 970~0.735 800μg、0.205 400~4.108 000μg、0.014 130~0.282 600μg、0.004 853~0.097 060μg;线性方程分别是Y=2 513.700X-18.223(r=0.998 7),Y=2 165.500X+40.317(r=0.999 8),Y=2 480.400X+0.768(r=0.999 8),Y=3 845.800X+0.778(r=0.999 9)。结论:本试验建立的方法稳定可靠、简便、重复性好、准确度高,可为补肾健骨口服液的含量测定提供参考依据。Objective:To establish an HPLC method for simultaneous determination of gallic acid,stilbene glucoside,emodin-8-O-β-D-glucoside and emodin in Bushen Jiangu Oral Liquid(补肾健骨口服液).Methods:HPLC was used,with Shim pack GIST C_(18)(250 mmx4.6 mm,5μm)as the chromatographic column.The mobile phase was acetonitrile 0.1%phosphoric acid solution,subjected to gradient elution.The column temperature was 30℃.The injection volume was 10μL.The detection wavelength was 285 nm.Results:The linear relationship of the 4 constituents was good in their respective linear range.The linear relationship was good in the concentra-tion range of 0.036970~0.735800μg,0.205400~4.108000μg,0.014130~0.282600μg,0.004853~0.097060μg.The standard curve equations of gallic acid,stilbene glucoside,emodin-8-O-β-D-glucoside and emodin were Y=2513.700X-18.223(r=0.9987),Y=2165.500X+40.317(r=0.9998),Y=2480.400X+0.768(r=0.9998),Y=3845.800X+0.778(r=0.9999).Conclusion:The method is stable,reliable,simple,reproducible and accurate.It can provide reference for the content determination of Bushen Jiangu Oral Liquid(补肾健骨服液).

关 键 词：补肾健骨口服液 高效液相色谱法 含量测定 没食子酸 二苯乙烯苷 大黄素-8-O-β-D-葡萄糖苷 大黄素 

分 类 号：R284.1[医药卫生—中药学]