老年急性脑梗死患者血清circTTC3和miR-138-5p水平变化及意义  

Changes and significance of serum circTTC3 and miR-138-5p levels in elderly patients with acute cerebral infarction

作　　者：古红 张奕玲[2] 赵宇[2] 张婷[3] 沈恺[4] Gu Hong;Zhang Yiling;Zhao Yu;Zhang Ting;Shen Kai(Geriatrics Center,West China Hospital of Sichuan University,Sichuan Province,Chengdu 610000,China;不详)

机构地区：[1]四川大学华西医院老年医学中心/干部医疗科,成都610000 [2]四川大学华西医院神经内科,成都610000 [3]四川大学华西医院老年内科,成都610000 [4]四川大学华西医院血液科,成都610000

出　　处：《疑难病杂志》2024年第5期537-541,547,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：四川省自然科学基金资助项目(2022NSFSC1463)。

摘　　要：目的探究老年急性脑梗死(ACI)患者血清环状RNA TTC3(circTTC3)和微小RNA-138-5p(miR-138-5p)水平变化及意义。方法选取2021年9月—2023年9月四川大学华西医院老年医学中心/干部医疗科诊治老年ACI患者128例(ACI组),根据美国国立卫生研究院卒中量表(NIHSS)分为轻度亚组42例、中度亚组49例和重度亚组37例,同期医院门诊体检的健康人员120例作为健康对照组;采用实时荧光定量PCR法测定血清circTTC3和miR-138-5p表达水平,Pearson法分析老年ACI患者血清circTTC3和miR-138-5p的相关性,Spearman法分析老年ACI患者血清circTTC3和miR-138-5p表达水平与NIHSS评分相关性,受试者工作特征(ROC)曲线分析血清circTTC3和miR-138-5p表达水平对老年ACI患者神经缺损程度的诊断价值。结果与健康对照组比较,ACI组血清circTTC3表达水平升高,miR-138-5p降低(t/P=17.207/<0.001、16.239/<0.001)。血清circTTC3水平比较,轻度亚组<中度亚组<重度亚组,miR-138-5p表达水平比较,轻度亚组>中度亚组>重度亚组(F/P=26.579/<0.001、105.935/<0.001)。老年ACI患者血清circTTC3表达水平与NIHSS评分呈正相关(r=0.521,P<0.001),与大脑中动脉(MCA)、大脑前动脉(ACA)血流速度呈负相关(r=-0.425、-0.392,P均<0.001);miR-138-5p表达水平与NIHSS评分呈负相关(r=-0.785,P<0.001),与MCA、ACA血流速度呈正相关(r=0.571、0.635,P均<0.001)。circTTC3、miR-138-5p及二者联合诊断ACI神经缺损程度的AUC分别为0.817、0.810、0.878,二者联合诊断价值优于单独诊断(Z/P=2.106/0.035、2.406/0.016)。结论老年ACI患者血清circTTC3表达水平升高,miR-138-5p表达水平降低,二者与患者病情的严重程度密切相关,可能作为ACI病情诊断、治疗相关的作用靶点。Objective To investigate the changes and significance of serum cyclic RNA TTC3(circTTC3)and microRNA-138-5p(miR-138-5p)levels in elderly patients with acute cerebral infarction(ACI).Methods From September 2021 to September 2023,the Geriatric Medicine Center/Cadre Medical Department of West China Hospital of Sichuan University treated 128 elderly patients with ACI(ACI group),according to the National Institute of Health Stroke Scale(NIHSS),elderly ACI patients were separated into a mild group of 42 cases,a moderate group of 49 cases,and a severe group of 37 cases,meantime,120 healthy individuals who came to the outpatient clinic for physical examination were regarded as a control group,real-time fluorescence quantitative PCR method was applied to determine the expression levels of circTTC3 and miR-138-5p in serum,Pearson method was applied to analyze the correlation between serum circTTC3 and miR-138-5p in elderly ACI patients,Spearman method was applied to analyze the correlation between serum circTTC3 and miR-138-5p levels and NIHSS scores in elderly ACI patients,ROC curve was applied to analyze the diagnostic value of serum circTTC3 and miR-138-5p expression levels in elderly ACI patients.Results Compared with the control group,the expression level of circTTC3 in serum of ACI group was increased,and miR-138-5p was decreased(t/P=17.207/<0.001,16.239/<0.001).The serum circTTC3 level in mild subgroup was lower than that in moderate subgroup than that in severe subgroup,and the expression level of miR-138-5p in mild subgroup was higher than that in moderate subgroup than that in severe subgroup(F/P=26.579/<0.001,105.935/<0.001).Serum circTTC3 expression level in elderly ACI patients was positively correlated with NIHSS score(r=0.521,P<0.001),and negatively correlated with MCA and ACA(r=-0.425,-0.392,all P<0.001).The expression level of miR-138-5p was negatively correlated with NIHSS score(r=-0.785,P<0.001),and positively correlated with MCA and ACA(r=0.571,0.635,all P<0.001).The AUC of circTTC3,miR-138-5p and combi

关 键 词：急性脑梗死 环状RNA TTC3 微小RNA-138-5p 神经功能 老年人 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R446.112[医药卫生—临床医学]