FZD7、GAL-8在肝内胆管癌中的表达及其与临床病理特征和预后的关系  

The expression of FZD7 and GAL-8 in intrahepatic cholangiocarcinoma and their relationship with clinical pathological features and prognosis

作　　者：马彦娥[1] 贺双 刘东梅[1] 苏虎艳 王倩如 郝光军[1] Ma Yan'e;He Shuang;Liu Dongmei;Su Huyan;Wang Qianru;Hao Guangjun(Department of Oncology,Yulin First Hospital,Shaanxi Province,Yulin 719000,China)

机构地区：[1]陕西省榆林市第一医院肿瘤科,719000

出　　处：《疑难病杂志》2024年第5期590-594,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省科技计划项目(2019M042)。

摘　　要：目的分析肝内胆管癌(ICC)中卷曲同源蛋白7(FZD7)、半乳糖凝集素8(GAL-8)的表达及其与临床病理特征和预后的关系。方法选取2017年4月—2020年4月榆林市第一医院肿瘤科诊治的ICC患者84例作为研究对象,患者均进行根治性手术。采用免疫组织化学法检测癌组织和癌旁组织FZD7、GAL-8蛋白表达;Spearman秩相关分析FZD7与GAL-8的相关性;Kaplan-Meier法分析FZD7、GAL-8表达对ICC患者生存预后的影响;Cox回归分析ICC的预后影响因素。结果ICC癌组织中FZD7、GAL-8阳性率分别为73.81%(62/84)、71.43%(60/84),高于癌旁组织的4.76%(4/84)、7.14%(6/84),差异有统计学意义(χ^(2)=83.950,72.770,P均<0.001);ICC中FZD7与GAL-8表达呈正相关(r s=0.745,P<0.001);低分化程度、有淋巴结转移、TNM分期Ⅲ期的ICC癌组织中FZD7、GAL-8阳性率高于高中分化、无淋巴结转移、TNM分期Ⅰ~Ⅱ期(FZD7:χ^(2)/P=8.221/<0.001,6.097/0.014,13.014/<0.001;GAL-8:χ^(2)/P=7.207/0.007,5.555/0.018,11.760/0.001)。FZD7阳性组3年总生存率为32.26%(20/62),低于阴性组的68.19%(15/22)(Log rankχ^(2)=8.723,P=0.003);GAL-8阳性组3年总生存率为28.33%(17/60),低于GAL-8阴性组的75.30%(18/24)(Log rankχ^(2)=24.310,P<0.001)。TNM分期Ⅲ期、低分化程度、淋巴结转移、FZD7阳性、GAL-8阳性是影响ICC患者预后的独立危险因素[HR(95%CI)=1.614(1.215~2.145),1.516(1.219~1.884),1.916(1.315~2.791),1.826(1.222~2.729),1.737(1.237~2.438)]。结论ICC癌组织中FZD7、GAL-8表达上调,两者均参与ICC肿瘤进展,是评估ICC患者预后的肿瘤标志物。Objective To analyze the expression of Frizzled homolog protein 7(FZD7)and galactin 8(GAL-8)in intrahepatic cholangiocarcinoma(ICC),and their relationship with clinical pathological features and prognosis.Methods Eighty-four ICC patients who visited Department of Oncology Yulin First Hospital from April 2017 to April 2020 were selected.Immunohistochemical methods were used to detect the expression of FZD7 and GAL-8 proteins in tissues.The relationship between FZD7 and GAL-8 were conducted by Spearman correlation analysis.Kaplan-Meier method was used to analyze the differences in survival prognosis among ICC patients with different FZD7 and GAL-8 expressions.The prognostic factors for ICC were analyzed by Cox regression analysis.Results The positive rates of FZD7 and GAL-8 in ICC cancer tissue were 73.81%(62/84)and 71.43%(60/84),respectively,which were higher than those in adjacent cancer tissue by 4.76%(4/84)and 7.14%(6/84)(χ^(2)=83.950,72.770,all P<0.001).FZD7 was positively correlated with GAL-8 expression in ICC(r s=0.745,P<0.001).The positive rates of FZD7 and GAL-8 were higher in ICC cancer tissues with low differentiation degree,lymph node metastasis and TNM stageⅢ(FZD7:χ^(2)/P=8.221/<0.001,6.097/0.014,13.014/<0.001;GAL-8:χ^(2)/P=7.207/0.007,5.555/0.018,11.760/0.001).The 3-year overall survival rate of the FZD7 positive group was 32.26%(20/62),which was lower than the negative group's 68.19%(15/22)(Log rankχ^(2)=8.723,P=0.003).The 3-year overall survival rate of the GAL-8 positive group was 28.33%(17/60),which was lower than the GAL-8 negative group's 75.30%(18/24)(Log rankχ^(2)=24.310,P<0.001).TNM stageⅢ,low differentiation degree,lymph node metastasis,FZD7 positivity,and GAL-8 positivity were independent risk factors affecting the prognosis of ICC patients[HR(95%CI)=1.614(1.215-2.145),1.516(1.219-1.884),1.916(1.315-2.791),1.826(1.222-2.729),1.737(1.237-2.438)].Conclusion FZD7 and GAL-8 are upregulated in ICC,both of which are involved in the progression of ICC tumors and serve as tumor markers fo

关 键 词：肝内胆管癌 卷曲同源蛋白7 半乳糖凝集素8 预后 

分 类 号：R735.7[医药卫生—肿瘤]