血清lncRNA ATP1A1-AS1对胰腺癌的诊断价值及其对细胞增殖、凋亡和端粒酶活性的影响  

Diagnostic value of serum lncRNA ATP1A1-AS1 in pancreatic cancer and its effect on cell proliferation,apoptosis and telomerase activity

作　　者：崔巍[1] 周威 刘智化 刘成栋 CUI Wei;ZHOU Wei;LIU Zhihua;LIU Chengdong(The Affiliated Xuancheng Hospital of Wannan Medical College,Xuancheng People s Hospital,Xuancheng 242099,China)

机构地区：[1]皖南医学院附属宣城医院,安徽省宣城市人民医院,安徽宣城242099

出　　处：《空军军医大学学报》2024年第5期567-571,共5页Journal of Air Force Medical University

基　　金：安徽省临床医学研究转化专项(202204295107020059)。

摘　　要：目的探讨血清长链非编码RNA Na+/K+ATP酶A1亚基的反义RNA1(ATP1A1-AS1)对胰腺癌的诊断价值及其对胰腺癌细胞增殖、凋亡和端粒酶活性的影响。方法选取2020年4月至2023年10月在安徽省宣城市人民医院就诊的65例胰腺癌患者为研究对象,并选取同时期60例健康体检者作为对照,qRT-PCR检测两组患者血清中ATP1A1-AS1表达水平,受试者工作特征(ROC)曲线分析血清ATP1A1-AS1对胰腺癌的诊断价值。体外培养胰腺癌细胞PANC-1,通过转染ATP1A1-AS1过表达载体上调PANC-1细胞中ATP1A1-AS1表达,MTT检测上调ATP1A1-AS1表达对PANC-1细胞增殖的影响,流式细胞术检测上调ATP1A1-AS1表达对PANC-1细胞凋亡的影响,Western blotting检测上调ATP1A1-AS1表达对PANC-1细胞中细胞周期蛋白D1(CyclinD1)、细胞增殖抗原Ki-67、B淋巴细胞瘤2(Bcl-2)和B淋巴细胞瘤2相关蛋白(Bax)蛋白表达的影响,端粒酶重复序列扩增法检测上调ATP1A1-AS1表达对PANC-1细胞端粒酶活性的影响。结果与对照组比较,胰腺癌患者血清中ATP1A1-AS1的表达水平显著降低(P<0.05)。ROC曲线分析结果显示,血清ATP1A1-AS1诊断胰腺癌的灵敏度为83.08%,特异度为86.67%,ROC曲线下面积为0.900。ATP1A1-AS1表达上调,PANC-1细胞增殖能力、端粒酶活性及细胞CyclinD1、Ki-67、Bcl-2蛋白表达水平降低(P<0.05),凋亡率和Bax蛋白表达水平升高(P<0.05)。结论ATP1A1-AS1在胰腺癌患者血清中呈低表达,可能是胰腺癌诊断的潜在生物学标志物。上调ATP1A1-AS1表达可抑制胰腺癌细胞增殖并诱导细胞凋亡,这可能与调控CyclinD1、Ki-67、Bcl-2和Bax蛋白表达及抑制细胞端粒酶活性有关。Objective To investigate the diagnostic value of antisense RNA1(ATP1A1-AS1)of serum long non-coding RNA Na+/K+-ATPase A1 subunit on pancreatic cancer and its effect on the proliferation,apoptosis and telomerase activity of pancreatic cancer cells.Methods Sixty-five patients with pancreatic cancer who were treated in Xuancheng People s Hospital from April 2020 to October 2023 were selected as the research subjects,and 60 healthy subjects were selected as controls at the same period.qRT-PCR was used to detect ATP1A1-AS1 expression levels in serum of two groups of patients.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of serum ATP1A1-AS1 on pancreatic cancer.Pancreatic cancer cells PANC-1 were cultured in vitro,and the expression of ATP1A1-AS1 in PANC-1 cells was up-regulated by transfection of ATP1A1-AS1 overexpression vector.MTT was used to detect the effect of up-regulating ATP1A1-AS1 expression on the proliferation of PANC-1 cells.Flow cytometry was used to detect the effect of up-regulating ATP1A1-AS1 expression on the apoptosis of PANC-1 cells.Western blotting was used to detect the effect of up-regulating ATP1A1-AS1 expression on the expression of CyclinD1,Ki-67,B-cell lymphoma-2(Bcl-2)and Bcl-2-associated X protein(Bax)in PANC-1 cells.Telomerase repeat amplification protocol was used to detect the effect of up-regulating ATP1A1-AS1 expression on the telomerase activity of PANC-1 cells.Results Compared with the control group,the expression level of ATP1A1-AS1 in the serum of patients with pancreatic cancer was significantly reduced(P<0.05).The results of ROC curve analysis showed that the sensitivity of serum ATP1A1-AS1 in the diagnosis of pancreatic cancer was 83.08%,the specificity was 86.67%,and the area under the ROC curve was 0.900.After up-regulating ATP1A1-AS1 expression,the proliferation ability,telomerase activity,and the expression levels of CyclinD1,Ki-67,and Bcl-2 in PANC-1 cells were reduced(P<0.05),while the apoptosis rate and Bax protein expression level w

关 键 词：胰腺癌 ATP1A1-AS1 细胞增殖 细胞凋亡 端粒酶活性 

分 类 号：R735.9[医药卫生—肿瘤]