权重依赖荧光寿命成像检测甘油-水混合物黏度  

Weight-dependent Fluorescence Lifetime Imaging for Viscosity Detection in Glycerol-water Mixtures

作　　者：罗腾[1] 赵谊华 陆原 严伟[1] 屈军乐[1] LUO Teng;ZHAO Yi-Hua;LU Yuan;YAN Wei;QU Jun-Le(Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Shenzhen Key Laboratory of Photonics and Biophotonics,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China;The 6th Affiliated Hospital of Shenzhen University,Huazhong University of Science and Technology Union Shenzhen Hospital,Shenzhen 518060,China)

机构地区：[1]深圳大学物理与光电工程学院,深圳市光子学与生物光子学重点实验室,光电子器件与系统教育部广东省重点实验室,深圳518060 [2]深圳大学第六附属医院/华中科技大学协和深圳医院,深圳518060

出　　处：《生物化学与生物物理进展》2024年第5期1221-1230,共10页Progress In Biochemistry and Biophysics

基　　金：深圳市基础研究项目(JCYJ20190808111418696);国家自然科学基金(61975127,62375180,62335008);深圳大学医工交叉研究基金(2023YG010)资助。

摘　　要：目的基于荧光寿命成像技术,提出一种黏度检测的新方法,并评估不同权重荧光寿命在区分甘油-水混合物黏度的能力,从而提供对黏度区分的准确度和可靠性。方法采用电子学中的权重依赖原理,分别引入了振幅加权平均荧光寿命(τ_(m))和强度加权平均荧光寿命(τ_(i))。通过τ_(m)和τ_(i)检测甘油-水混合物黏度变化。振幅加权平均荧光寿命反映了荧光信号振幅与时间的关系,而强度加权平均荧光寿命则关注荧光信号强度的时变特性。结果τ_(m)和τ_(i)两种结果相互佐证,这不仅提高了对甘油-水混合物黏度变化的可靠性,同时也揭示了τ_(m)和τ_(i)在检测过程中的独特作用。尽管τ_(m)在捕捉荧光信号振幅变化方面发挥了关键作用,然而,τ_(i)在考虑荧光信号强度的时变特性时表现出更高的黏度检测准确度。特别值得注意的是,由于τ_(i)更为敏感,微环境黏度检测可以直接利用τ_(i)进行分析。这为实时、高灵敏度的微流体黏度监测提供了更为便捷的途径。通过对τ_(m)和τ_(i)的综合利用,不仅能够更全面、准确地了解甘油-水混合物的黏度信息,而且可以根据具体需求选择更为适用的参数进行深入分析。结论振幅加权和强度加权的结合可以在不同条件下更为灵敏地识别黏度的微小变化。这种方法的创新之处在于其对多参数的同时关注,提高了对黏度变化的敏感性和可分辨性。因此,这种权重依赖的荧光寿命成像技术不仅为甘油-水混合物的黏度探测提供了一种全新的方法,同时也为微流体、流变学以及新型功能材料研究等领域提供了有力的分析手段。Objective Based on fluorescence lifetime imaging technology,a novel method for viscosity detection is proposed and the capability of different weighting of fluorescence lifetimes in distinguishing the viscosity of glycerol-water mixtures is evaluated,aiming to enhance the accuracy and reliability of viscosity differentiation.Methods This approach incorporates the principles of electronic weighting,introducing both amplitude-weighted average fluorescence lifetime(τ_(m))and intensity-weighted average fluorescence lifetime(τ_(i)).Viscosity changes in glycerol-water mixtures are detected through τ_(m) and τ_(i).τ_(m) Reflects the relationship between fluorescence signal amplitude and time,whileτi focuses on the time-varying characteristics of fluorescence signal intensity.Results The results of both τ_(m) and τ_(i) mutually corroborate each other,not only enhancing the reliability in detecting viscosity changes in glycerol-water mixtures but also revealing their unique roles in the detection process.Although τ_(m) plays a crucial role in capturing changes in fluorescence signal amplitude,τ_(i) exhibits higher accuracy in viscosity detection when considering the time-varying characteristics of fluorescence signal intensity.It is particularly noteworthy that,due to τ_(i)’s greater sensitivity,microenvironment viscosity detection can be directly analyzed usingτi.This provides a more convenient approach for real-time,highly sensitive microfluidic viscosity monitoring.Therefore,through the comprehensive utilization of τ_(m) and τ_(i),a more thorough and accurate understanding of the viscosity information in glycerol-water mixtures can be obtained,and specific parameters can be selected for in-depth analysis based on specific needs.Conclusion The combination of amplitude weighting and intensity weighting allows for a more sensitive identification of subtle changes in viscosity under different conditions.The innovation of this method lies in its simultaneous consideration of multiple parameters,enhancing sen

关 键 词：荧光寿命 黏度 权重 甘油 

分 类 号：Q631[生物学—生物物理学] O439[机械工程—光学工程] TN911.74[理学—光学]