低温冷藏对夜蛾黑卵蜂生长发育的影响  

Effects of cold storage on growth and development of Telenomus remus

作　　者：盛龙玉 杨文俊 毛燕 王增霞 黄保宏 SHENG Longyu;YANG Wenjun;MAO Yan;WANG Zengxia;HUANG Baohong(College of Resource and Environment,Anhui Science and Technology University,Fengyang 233100,China;College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院资源与环境学院,安徽凤阳233100 [2]安徽科技学院农学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2024年第3期42-47,共6页Journal of Anhui Science and Technology University

基　　金：安徽省重点研发计划(202104a06020002);国家重点研发计划(2021YFD1400701);安徽省高校自然科学研究项目(2022AH051654)。

摘　　要：目的:明确夜蛾黑卵蜂的最佳冷藏方式,获得持续稳定且数量充足的草地贪夜蛾天敌,满足生物防治的需要。方法:通过设置低温驯化、不同冷藏温度及冷藏时间等处理,探究不同冷藏方式对夜蛾黑卵蜂亲代和子代羽化率、发育历期及雌性比的影响。结果:不同冷藏温度及冷藏时间对夜蛾黑卵蜂亲代和子代生长发育均有显著影响。经过低温驯化后,夜蛾黑卵蜂亲代和子代的羽化率比直接冷藏的羽化率高,且经过低温冷藏后,雌性比有一定提高,但发育历期明显延长。因此,在生产中推荐合适的冷藏条件为低温驯化后,亲代夜蛾黑卵蜂在10℃下冷藏5、8 d,子代夜蛾黑卵蜂在4℃下冷藏3、5 d或在10℃下冷藏5、8 d。结论:该研究结果为夜蛾黑卵蜂低温贮藏技术提供科学依据,以便更好地应用于生物防治。Objective:To define the optimal cold storage conditions for Telenomus remus,to obtain a stable and sufficient number of parasitoids and meet the needs of biological control.Methods:By setting cold acclimation,different cold storage temperatures,and cold storage times,this study investigates the effects of different cold storage methods on the emergence rate,developmental duration,and female ratio of parents and offspring of Telenomus remus.Results:Different cold storage temperatures and durations have significant effects on the growth and development of parents and offspring of Telenomus remus.After cold acclimation,the emergence rate of parents and offspring of Telenomus remus was higher than that of direct cold storage,and after cold acclimation,the female ratio was slightly improved,but the development period was significantly prolonged.Based on these results,we recommend the following cold storage protocols for producation after cold acclimation,the parent Telenomus remus is storaged at 10℃for 5,8 d,and the offspring Telenomus remus is storaged at 4℃for 3,5 d or at 10℃for 5,8 d.Conclusion:The research results provide scientific basis for the cold storage technology of Telenomus remus,in order to better apply it to biological control.

关 键 词：夜蛾黑卵蜂 草地贪夜蛾 低温驯化 低温冷藏 羽化率 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]